Product nameAnti-CNTF antibody (Biotin)
See all CNTF primary antibodies
DescriptionRabbit polyclonal to CNTF (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Rat
Recombinant fragment corresponding to Rat CNTF aa 2-200.
AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLD SVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPT EGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGL FEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM
Database link: P20294
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionCNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Tissue specificityNervous system.
Sequence similaritiesBelongs to the CNTF family.
- Information by UniProt
- Ciliary neurotrophic factor antibody
- CNTF antibody
- CNTF_HUMAN antibody
- HCNTF antibody
Western blot analysis of varying levels of Recombinant rat CNTF protein using ab271273 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Rat CNTF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271273. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat CNTF protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271273 has not yet been referenced specifically in any publications.