Overview

  • Product name

    Anti-CNTFR antibody [MM0190-10L29]
    See all CNTFR primary antibodies
  • Description

    Mouse monoclonal [MM0190-10L29] to CNTFR
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CNTFR.

Properties

Applications

Our Abpromise guarantee covers the use of ab89333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells.

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.

Target

  • Function

    Binds to CNTF. The alpha subunit provides the receptor specificity.
  • Tissue specificity

    Nervous system and skeletal muscle.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ciliary neurotrophic factor receptor alpha antibody
    • Ciliary neurotrophic factor receptor antibody
    • Ciliary neurotrophic factor receptor subunit alpha antibody
    • CNTF Receptor alpha antibody
    • CNTF receptor subunit alpha antibody
    • CNTFR Alpha antibody
    • CNTFR antibody
    • CNTFR-alpha antibody
    • CNTFR_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CNTFR with ab89333. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

  • Overlay histogram showing SH-SY5Y cells stained with ab89333 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89333, 0.5µg/1x106 cells ) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2 (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

ab89333 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate, Placenta)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Prostate, Placenta
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 75% · Temperature: rt°C
Fixative
Paraformaldehyde

Dr. giovanni tossetta

Verified customer

Submitted Feb 22 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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