Key features and details
- Rabbit polyclonal to CNTLN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CNTLN antibody
DescriptionRabbit polyclonal to CNTLN
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- Human liver tissue; Human liver lysate; Human cell line U-251 MG
Previously labelled as Centlein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.|
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase.
- Information by UniProt
- bA340N12.1 antibody
- C9orf101 antibody
- C9orf39 antibody
ab122356, at 1/60 dilution, staining CNTLN in paraffin-embedded Human liver tissue by Immunohistochemistry.
All lanes : Anti-CNTLN antibody (ab122356) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
ab122356, at 4 µg/ml, staining CNTLN in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.
ab122356 has not yet been referenced specifically in any publications.