Overview

  • Product name

    Anti-CNTLN antibody
  • Description

    Rabbit polyclonal to CNTLN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CNTLN aa 289-379.
    Sequence:

    LIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLY KQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESIS

  • Positive control

    • Human liver tissue; Human liver lysate; Human cell line U-251 MG
  • General notes

    Previously labelled as Centlein.

Properties

Applications

Our Abpromise guarantee covers the use of ab122356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA340N12.1 antibody
    • C9orf101 antibody
    • C9orf39 antibody
    • Centlein antibody
    • Centlein centrosomal protein antibody
    • Centrosomal protein antibody
    • Cntln antibody
    • CNTLN_HUMAN antibody
    • FLJ20276 antibody
    • RP11-340N12.1 antibody
    see all

Images

  • ab122356, at 1/60 dilution, staining CNTLN in paraffin-embedded Human liver tissue by Immunohistochemistry.

  • All lanes : Anti-CNTLN antibody (ab122356) at 1/250 dilution

    Lane 1 : RT-4 cell lysate

    Lane 2 : U-251 MG cell lysate

    Lane 3 : Human plasma lysate

    Lane 4 : Human liver lysate

    Lane 5 : Human tonsil lysate
  • ab122356, at 4 µg/ml, staining CNTLN in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.

References

ab122356 has not yet been referenced specifically in any publications.

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