Anti-COASY antibody (ab155551)

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Western blot - Anti-COASY antibody (ab155551)
  • Immunocytochemistry/ Immunofluorescence - Anti-COASY antibody (ab155551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COASY antibody (ab155551)

Key features and details

  • Rabbit polyclonal to COASY
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-COASY antibody
    See all COASY primary antibodies

  • Description

    Rabbit polyclonal to COASY

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-291 of Human COASY (Uniprot ID: Q13057).

  • Positive control

    • HeLa whole cell lysate; HeLa cells; colon carcinoma tissue.

  • General notes

    Keep as concentrated solution.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.

  • Tissue specificity

    Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.

  • Pathway

    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.

  • Sequence similarities

    In the central section; belongs to the eukaryotic coaD family.
    Contains 1 DPCK (dephospho-CoA kinase) domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    Images

    • Western blot - Anti-COASY antibody (ab155551)
      Western blot - Anti-COASY antibody (ab155551)
      Anti-COASY antibody (ab155551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 62 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-COASY antibody (ab155551)
      Immunocytochemistry/ Immunofluorescence - Anti-COASY antibody (ab155551)
      Immunofluorescence analysis of methanol-fixed HeLa cells labeling COASY with ab155551 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COASY antibody (ab155551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COASY antibody (ab155551)
      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling COASY with ab155551 at 1/500 dilution.

    References (0)

    Publishing research using ab155551? Please let us know so that we can cite the reference in this datasheet.

    ab155551 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-COASY antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    Submitted Oct 17 2016

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