Overview

  • Product name
  • Description
    Rabbit polyclonal to COASY
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-291 of Human COASY (Uniprot ID: Q13057).

  • Positive control
    • HeLa whole cell lysate; HeLa cells; colon carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
  • Tissue specificity
    Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
  • Pathway
    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
  • Sequence similarities
    In the central section; belongs to the eukaryotic coaD family.
    Contains 1 DPCK (dephospho-CoA kinase) domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional coenzyme A synthase antibody
    • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
    • CoA synthase antibody
    • COASY antibody
    • COASY_HUMAN antibody
    • Coenzyme A synthase antibody
    • Dephospho-CoA kinase antibody
    • Dephospho-CoA pyrophosphorylase antibody
    • Dephosphocoenzyme A kinase antibody
    • DPCK antibody
    • DPCOAK antibody
    • NBP antibody
    • Nucleotide binding protein antibody
    • Pantetheine-phosphate adenylyltransferase antibody
    • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
    • pOV 2 antibody
    • POV-2 antibody
    • POV2 antibody
    • PPAT antibody
    • UKR1 antibody
    see all

Images

  • Anti-COASY antibody (ab155551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling COASY with ab155551 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling COASY with ab155551 at 1/500 dilution.

References

ab155551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Oct 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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