Anti-COASY antibody (ab155551)
Key features and details
- Rabbit polyclonal to COASY
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COASY antibody
See all COASY primary antibodies -
Description
Rabbit polyclonal to COASY -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-291 of Human COASY (Uniprot ID: Q13057).
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Positive control
- HeLa whole cell lysate; HeLa cells; colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. -
Tissue specificity
Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain. -
Pathway
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5. -
Sequence similarities
In the central section; belongs to the eukaryotic coaD family.
Contains 1 DPCK (dephospho-CoA kinase) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 80347 Human
- Omim: 609855 Human
- SwissProt: Q13057 Human
- Unigene: 296422 Human
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Alternative names
- Bifunctional coenzyme A synthase antibody
- Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
- CoA synthase antibody
see all
Images
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Western blot - Anti-COASY antibody (ab155551)Anti-COASY antibody (ab155551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-COASY antibody (ab155551)Immunofluorescence analysis of methanol-fixed HeLa cells labeling COASY with ab155551 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COASY antibody (ab155551)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling COASY with ab155551 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155551 has not yet been referenced specifically in any publications.