Anti-COASY antibody (ab226400)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-COASY antibody
See all COASY primary antibodies -
Description
Rabbit polyclonal to COASY -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide within Human COASY aa 225-275. The exact sequence is proprietary.
Database link: Q13057 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
ab226400 has not performed satisfactorily when used for WB of COASY in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of COASY.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226400 was affinity purified using an epitope specific to COASY immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. -
Tissue specificity
Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain. -
Pathway
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5. -
Sequence similarities
In the central section; belongs to the eukaryotic coaD family.
Contains 1 DPCK (dephospho-CoA kinase) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 80347 Human
- Entrez Gene: 396688 Pig
- Omim: 609855 Human
- SwissProt: Q13057 Human
- SwissProt: Q8MIR4 Pig
- Unigene: 296422 Human
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Alternative names
- Bifunctional coenzyme A synthase antibody
- Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
- CoA synthase antibody
see all
Images
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Immunoprecipitation - Anti-COASY antibody (ab226400)COASY was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226400 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226400 at 1 µg/ml.
Lane 1: ab226400 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Datasheets and documents
References
ab226400 has not yet been referenced specifically in any publications.
