Overview

  • Product name

  • Description

    Rabbit polyclonal to COASY
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment within Human COASY (internal sequence). The exact sequence is proprietary.
    Database link: Q13057

  • Positive control

    • WB: Mouse liver tissue lysate; HeLa S3 whole cell lysate. IHC-P: Mouse kidney and prostate tissue; NCIN87 xenograft tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.

Target

  • Function

    Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
  • Tissue specificity

    Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
  • Pathway

    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
  • Sequence similarities

    In the central section; belongs to the eukaryotic coaD family.
    Contains 1 DPCK (dephospho-CoA kinase) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bifunctional coenzyme A synthase antibody
    • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
    • CoA synthase antibody
    • COASY antibody
    • COASY_HUMAN antibody
    • Coenzyme A synthase antibody
    • Dephospho-CoA kinase antibody
    • Dephospho-CoA pyrophosphorylase antibody
    • Dephosphocoenzyme A kinase antibody
    • DPCK antibody
    • DPCOAK antibody
    • NBP antibody
    • Nucleotide binding protein antibody
    • Pantetheine-phosphate adenylyltransferase antibody
    • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
    • pOV 2 antibody
    • POV-2 antibody
    • POV2 antibody
    • PPAT antibody
    • UKR1 antibody
    see all

Images

  • Anti-COASY antibody (ab227272) at 1/3000 dilution + Mouse liver tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded mouse kidney tissue stained for COASY with ab227272 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for COASY (green) using ab227272 at 1/200 dilution in ICC/IF.

    Blue: Hoechst 33342 staining (lower panel).

  • Paraffin-embedded mouse prostate tissue stained for COASY with ab227272 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded NCIN87 xenograft tissue stained for COASY with ab227272 at 1/500 dilution in immunohistochemical analysis.

  • Anti-COASY antibody (ab227272) at 1/1000 dilution + HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    7.5% SDS-PAGE

References

ab227272 has not yet been referenced specifically in any publications.

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