Recombinant
RabMAb

Recombinant Anti-COASY antibody [EPR8246] (ab129012)

Overview

  • Product name

    Anti-COASY antibody [EPR8246]
    See all COASY primary antibodies
  • Description

    Rabbit monoclonal [EPR8246] to COASY
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human COASY aa 550-650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, HeLa and A431 cell lysates; permeabilized HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
    • Tissue specificity

      Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
    • Pathway

      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
    • Sequence similarities

      In the central section; belongs to the eukaryotic coaD family.
      Contains 1 DPCK (dephospho-CoA kinase) domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    Images

    • All lanes : Anti-COASY antibody [EPR8246] (ab129012) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 62 kDa

    • ab129012, at 1/10 dilution, staining COASY in permeabilized HeLa cells by Flow Cytometry (red) or a Rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129012 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Nov 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up