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    coasy-antibody-epr8246-bsa-and-azide-free-ab248259.pdf

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RecombinantRabMAb

Recombinant Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
  • Flow Cytometry (Intracellular) - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
  • OI-RD Scanning - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
  • Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8246] to COASY - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-COASY antibody [EPR8246] - BSA and Azide free
    See all COASY primary antibodies
  • Description

    Rabbit monoclonal [EPR8246] to COASY - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248259 is the carrier-free version of ab129012.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 4.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8246
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-COASY antibody [EPR8246] (ab129012)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human COASY protein (ab211940)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
  • Tissue specificity

    Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
  • Pathway

    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
    Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
  • Sequence similarities

    In the central section; belongs to the eukaryotic coaD family.
    Contains 1 DPCK (dephospho-CoA kinase) domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13057 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 80347 Human
    • Omim: 609855 Human
    • SwissProt: Q13057 Human
    • Unigene: 296422 Human
    • Alternative names

      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    Images

    • Western blot - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      Western blot - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      All lanes : Anti-COASY antibody [EPR8246] (ab129012) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 62 kDa



      This data was developed using ab129012, the same antibody clone in a different buffer formulation.

    • Flow Cytometry (Intracellular) - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      Flow Cytometry (Intracellular) - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)

      This data was developed using ab129012, the same antibody clone in a different buffer formulation. 

      ab129012, at 1/10 dilution, staining COASY in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green).

       

    • OI-RD Scanning - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      OI-RD Scanning - Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      This data was developed using ab129012, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)
      Anti-COASY antibody [EPR8246] - BSA and Azide free (ab248259)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab248259? Please let us know so that we can cite the reference in this datasheet.

    ab248259 has been referenced in 1 publication.

    • van Dijk T  et al. Biallelic loss of function variants in COASY cause prenatal onset pontocerebellar hypoplasia, microcephaly, and arthrogryposis. Eur J Hum Genet 26:1752-1758 (2018). PubMed: 30089828

    Customer reviews and Q&As

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