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    cobalt-assay-kit-ab102510.pdf

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Cobalt Assay Kit (ab102510)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - Cobalt Assay Kit (ab102510)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 20 min
    • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
    • Sensitivity: 0.18 µg

    Overview

    • Product name

      Cobalt Assay Kit
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
    • Assay type

      Quantitative
    • Sensitivity

      > 0.18 µg
    • Range

      0.18 µg - 3 µg
    • Assay time

      0h 20m
    • Product overview

      Abcam's Cobalt Assay Kit takes advantage of the reaction of Co2+ with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe2+, Cu2+, Ni2+, Zn2+, Mn2+, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co2+ in a variety of samples.
      Visit our FAQs page for tips and troubleshooting.

    • Notes

      Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co2+ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 100 tests
      Cobalt Chloride Standard Yellow 1 unit
      Cobalt Reagent Red 1 x 1ml
    • Research areas

      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cell Biology
      • Other Antibodies
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    • Relevance

      Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co+2 species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range.
    • Alternative names

      • Co

    Images

    • Functional Studies - Cobalt Assay Kit (ab102510)
      Functional Studies - Cobalt Assay Kit (ab102510)
      Cobalt Standard Curve: Assays were performed following the kit protocol.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab102510? Please let us know so that we can cite the reference in this datasheet.

    ab102510 has not yet been referenced specifically in any publications.

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