Key features and details
- Rabbit polyclonal to Cobra Venom Factor
- Suitable for: WB
- Isotype: IgG
Product nameAnti-Cobra Venom Factor antibody
DescriptionRabbit polyclonal to Cobra Venom Factor
Tested applicationsSuitable for: WBmore details
Predicted to work with: Monocled cobra
Recombinant fragment corresponding to Monocled cobra Cobra Venom Factor aa 733-984.
DDNEDGFIADSDIISRSDFPKSWLWLTKDLTEEPNSQGISSKTMSFYLRD SITTWVVLAVSFTPTKGICVAEPYEIRVMKVFFIDLQMPYSVVKNEQVEI RAILHNYVNEDIYVRVELLYNPAFCSASTKGQRYRQQFPIKALSSRAVPF VIVPLEQGLHDVEIKASVQEALWSDGVRKKLKVVPEGVQKSIVTIVKLDP RAKGVGGTQLEVIKARKLDDRVPDTEIETKIIIQGDPVAQIIENSIDGSK LN
Database link: Q91132
- WB: Cobra Venom Factor recombinant protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 185 kDa.
This protein is 185kDa before any post-translational modifications, but it can be split into 3 chains: alpha (aa 23-649), beta (aa 1264-1642) and gamma (aa 733-984). The immunogen correspond to the gamma chain. Database link: Q91132
All lanes : Anti-Cobra Venom Factor antibody (ab229942) at 1/500 dilution
Lane 1 : Cobra Venom Factor recombinant protein at 0.08 µg
Lane 2 : Cobra Venom Factor recombinant protein at 0.04 µg
Lane 3 : Cobra Venom Factor recombinant protein at 0.02 µg
Lane 4 : Cobra Venom Factor recombinant protein at 0.01 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 185 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
This protein is 185kDa before any post-translational modifications, but it can be split into 3 chains: alpha (aa 23-649), beta (aa 1264-1642) and gamma (aa 733-984). ab229942 detects the gamma chain.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229942 has not yet been referenced specifically in any publications.