Key features and details
- Rabbit polyclonal to CoCoA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CoCoA antibody
See all CoCoA primary antibodies
DescriptionRabbit polyclonal to CoCoA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within internal sequence amino acids 202-496 of Human CoCoA (Uniprot ID: Q9P1Z2).
- HeLaS3 whole cell lysaate; Human C2C12 xenograft tissue; HeLa cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 77 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
RelevanceFunctions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions.
Cellular localizationCytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm.
- Calcium binding and coiled coil domain 1 antibody
- CALCOCO1 antibody
- Calphoglin antibody
Anti-CoCoA antibody (ab154731) at 1/500 dilution + HeLaS3 whole cell lysate at 30 µg
Predicted band size: 77 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells (ice-cold; 5 mins) labeling CoCoA with ab154731 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft tissue labeling CoCoA with ab154731 at 1/100 dilution.
ab154731 has not yet been referenced specifically in any publications.