Coelenterazine, native, Aequorin luminophore (ab145165)

Overview

  • Product name
    Coelenterazine, native, Aequorin luminophore
  • Description
    Cell-permeable aequorin luminophore. Chemiluminescent superoxide anion indicator.
  • Biological description
    Cell-permeable aequorin luminophore. Chemiluminescent superoxide anion indicator. Gaussia luciferase substrate. Detects changes in intracellular Ca2+ in apoaequorin transfected cells. Emits blue light for in vivo imaging.
  • CAS Number
    55779-48-1
  • Chemical structure
    Chemical Structure

Properties

References

This product has been referenced in:
  • Inouye S  et al. Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines. Protein Expr Purif 88:150-6 (2013). Read more (PubMed: 23274053) »
  • Stacer AC  et al. NanoLuc reporter for dual luciferase imaging in living animals. Mol Imaging 12:1-13 (2013). Read more (PubMed: 24371848) »
  • Kim SB  et al. Superluminescent variants of marine luciferases for bioassays. Anal Chem 83:8732-40 (2011). Read more (PubMed: 21951281) »

Customer reviews and Q&As

Question
Answer



I can confirm that ab145165 (Coelenterazine, native) is oxidized by Renilla luciferase, an enzyme derived from the sea pansy, and this reaction can be used to measure a variety of activities in cellular or animal models when luciferase is used as a reporter gene. Coelenterazine is one of two components of aequorin, a calcium-sensitive photoprotein found in bioluminescent jellyfish.

I hope this information is helpful when deciding if this product is suitable for your customers' experiments.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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