Coenzyme A Assay Kit (ab102504)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2.5 µM
Overview
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Product name
Coenzyme A Assay Kit
See all Coenzyme A kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 2.5 µM -
Range
2.5 µM - 250 µM -
Assay time
1h 00m -
Product overview
Abcam's Coenzyme A Assay Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K367 Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit. K367-100 is the same size as the 100 test size of ab102504.
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ACS Substrate 1 x 1ml Acyl CoA Enzyme Mix 1 vial Assay Buffer V 1 x 25ml CoA Converter Mix 1 unit CoA Standard 1 unit OxiRed Probe 1 unit -
Research areas
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Relevance
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. -
Alternative names
- CoA
- CoASH
- HSCoA
Images
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab102504 has been referenced in 6 publications.
- Chang X et al. Pilot trial on the efficacy and safety of pantethine in children with pantothenate kinase-associated neurodegeneration: a single-arm, open-label study. Orphanet J Rare Dis 15:248 (2020). PubMed: 32928263
- Le CH et al. Tafazzin deficiency impairs CoA-dependent oxidative metabolism in cardiac mitochondria. J Biol Chem 295:12485-12497 (2020). PubMed: 32665401
- Smith ER et al. TGF-ß1 modifies histone acetylation and acetyl-coenzyme A metabolism in renal myofibroblasts. Am J Physiol Renal Physiol N/A:N/A (2019). PubMed: 30623724
- Dieterich IA et al. Acetyl-CoA flux regulates the proteome and acetyl-proteome to maintain intracellular metabolic crosstalk. Nat Commun 10:3929 (2019). PubMed: 31477734
- Iuso A et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). PubMed: 29754768
- Machado MV et al. Vitamin B5 and N-Acetylcysteine in Nonalcoholic Steatohepatitis: A Preclinical Study in a Dietary Mouse Model. Dig Dis Sci 61:137-48 (2016). PubMed: 26403427