Overview

  • Product name

    Coenzyme A Assay Kit
    See all Coenzyme A kits
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Quantitative
  • Sensitivity

    > 2.5 µM
  • Range

    2.5 µM - 250 µM
  • Assay time

    1h 00m
  • Product overview

    Abcam's Coenzyme A Assay Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.

  • Platform

    Microplate reader

Properties

Images

  • Example of Standard Curve obtained using ab102504.

  • Example of Standard Curve obtained using ab102504.

Protocols

References

This product has been referenced in:

  • Iuso A  et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). Read more (PubMed: 29754768) »
  • Machado MV  et al. Vitamin B5 and N-Acetylcysteine in Nonalcoholic Steatohepatitis: A Preclinical Study in a Dietary Mouse Model. Dig Dis Sci 61:137-48 (2016). Read more (PubMed: 26403427) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for the information, and the clarification. I am not sure we have any data for precision, which would tell us how well the assay could distinguish among differences. The low standard, 0.2 nmol per well in the fluorescent version of the assay, is 5uM, in a volume of 40 ul, so it is conceivable the assay could distinguish between values that differ by 1uM in 75ul, if they are highly reproducible. I have asked the lab to confirm they have tested reproducibility and for their opinion.

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Answer

Thank you for your interest in the Coenzyme A assay, ab102504. The detection limit of the fluorescent assay is 2.5uM, so it will not be suitable for detection of your 1uM samples. I am waiting for the lab to confirm compatibility with the reducing agents you listed and will forward this information when I receive it. In case they ask, can you please tell me the concentrations of DTT/BME/TCEP you expect your samples will contain?

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Answer

Thank you for your inquiry. Unfortunately we have not specifically tested the compatibilities of these compounds with this kit, so we cannot say with certainty whether these will be compatible. The kit should provide up to 100 tests (includes standards). I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything fu

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Answer

Thank you for your follow up question. The lab producing this kit informed us that unfortunately the reaction mechanism information is proprietary and hence we cannot list all details except the one on the data sheet. Regarding the second part of your email, indeed, in the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe. The acyl CoA can generate background, as can long chain CoA. It is therefore necessary to do a background control. This control can be don by omitting the Conversion Enzyme from the reaction. The acyl-CoA background can then and should be subtracted from CoA readings. So the laboratory confirmed that with some optimizations for this sample type, you should be able to use this kit. Please let me know if you have any more questions.

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Answer

Thank you for your inquiry. Unfortunately we do not have any references for this product as yet. I would like however to reassure you that we do guarantee this product to work as stated on the datasheet. We do indeed guarantee all our products to work as stated on the datasheet (www.abcam.com/abpromise). Also, if you are using this product, we would like to encourage you to provide us with your feedback. You can simply send your feedback - ideally in form of a graph of the results with a short description - to us and we will also reward you with Abpoints (Abpoints can then be exchanged for reductions www.abcam.com/abpoints). I am sorry for not being able to provide references. I hope this information is nevertheless helpful. Please do not hesitate to contact us with further questions.

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