• Product name
    Coenzyme A Assay Kit
    See all Coenzyme A kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
  • Sensitivity
    > 2.5 µM
  • Range
    2.5 µM - 250 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Coenzyme A Assay Kit provides an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.



  • Example of Standard Curve obtained using ab102504.

  • Example of Standard Curve obtained using ab102504.



ab102504 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for the information, and the clarification. I am not sure we have any data for precision, which would tell us how well the assay could distinguish among differences. The low standard, 0.2 nmol per well in the fluorescent version of the assay,...

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Thank you for your interest in the Coenzyme A assay, ab102504. The detection limit of the fluorescent assay is 2.5uM, so it will not be suitable for detection of your 1uM samples. I am waiting for the lab to confirm compatibility with the reducing agen...

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Thank you for your inquiry. Unfortunately we have not specifically tested the compatibilities of these compounds with this kit, so we cannot say with certainty whether these will be compatible. The kit should provide up to 100 tests (in...

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Thank you for your follow up question. The lab producing this kit informed us that unfortunately the reaction mechanism information is proprietary and hence we cannot list all details except the one on the data sheet. Regarding the second...

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Thank you for your inquiry. Unfortunately we do not have any references for this product as yet. I would like however to reassure you that we do guarantee this product to work as stated on the datasheet. We do indeed guarantee all our products to w...

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