Product nameCoenzyme A Assay Kit (Fluorometric - Green)
See all Coenzyme A kits
Sample typeCell culture extracts, Tissue Extracts, Cell Lysate
Sensitivity= 40 nM
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
Coenzyme A Assay Kit (Fluorometric - Green) (ab138889) provides an ultrasensitive fluorometric assay to quantitate CoA content by detection of the –SH group in CoA.
The fluorogenic AcetylCoA green indicator dye used in the kit becomes strongly fluorescent upon reacting with –SH.
The assay kit can detect as little as 4 pmol CoA/ 100 µL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format at Ex/Em = 490/520 nm, and easily adapted to automation without a separation step.
Coenzyme A assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 10-60 min whilst measuring fluorescence with a microplate reader
Previously called Coenzyme A Detection kit (Fluorometric - Green).
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests AcetylCoA Green Indicator 1 vial Assay Buffer 1 x 25ml Coenzyme A Standard 1 x 154µg DMSO 1 x 200µl
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
RelevanceCoenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of Coenzyme A is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-Coenzyme A. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
This product has been referenced in:
- Iuso A et al. A Homozygous Splice Site Mutation in SLC25A42, Encoding the Mitochondrial Transporter of Coenzyme A, Causes Metabolic Crises and Epileptic Encephalopathy. JIMD Rep 44:1-7 (2019). Read more (PubMed: 29923093) »
- Iuso A et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). Read more (PubMed: 29754768) »