Overview

  • Product name

    Anti-Cofilin 1 + 2 antibody
  • Description

    Rabbit polyclonal to Cofilin 1 + 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 86-90 (

    ATYET

    ) of Human Cofilin 1 + 2 (NP_005498.1, NP_068733.1).

  • Positive control

    • Methanol-fixed HeLa cells; MDA cell extract; Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/200.
ICC/IF 1/50 - 1/100.

Target

  • Relevance

    Cofilin 1: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Cofilin 2: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Cellular localization

    Cofilin 1: Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. Cofilin 2: Nucleus matrix. Cytoplasm, cytoskeleton
  • Database links

  • Alternative names

    • 18 kDa phosphoprotein antibody
    • CFL antibody
    • Cofilin 1 (non-muscle) antibody
    • cofilin 1 antibody
    • Cofilin 2 (muscle) antibody
    • cofilin 2 antibody
    • Cofilin, muscle isoform antibody
    • Cofilin, non-muscle isoform antibody
    • NEM7 antibody
    • p18 antibody
    see all

Images

  • Anti-Cofilin 1 + 2 antibody (ab131519) at 1/500 dilution + MDA cell extract

    Predicted band size: 19 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.

References

This product has been referenced in:

  • Zhang Y  et al. Paclitaxel inhibits breast cancer metastasis via suppression of Aurora kinase-mediated cofilin-1 activity. Exp Ther Med 15:1269-1276 (2018). Read more (PubMed: 29434713) »
See 1 Publication for this product

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