Key features and details
- Rabbit polyclonal to Cofilin 1 + 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cofilin 1 + 2 antibody
DescriptionRabbit polyclonal to Cofilin 1 + 2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Methanol-fixed HeLa cells; MDA cell extract; Human breast carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 19 kDa.|
|IHC-P||1/100 - 1/200.|
|ICC/IF||1/50 - 1/100.|
RelevanceCofilin 1: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Cofilin 2: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Cellular localizationCofilin 1: Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. Cofilin 2: Nucleus matrix. Cytoplasm, cytoskeleton
- 18 kDa phosphoprotein antibody
- CFL antibody
- Cofilin 1 (non-muscle) antibody
Anti-Cofilin 1 + 2 antibody (ab131519) at 1/500 dilution + MDA cell extract
Predicted band size: 19 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.
ab131519 has been referenced in 2 publications.
- Galvan DL et al. Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice. J Clin Invest 129:2807-2823 (2019). PubMed: 31063459
- Zhang Y et al. Paclitaxel inhibits breast cancer metastasis via suppression of Aurora kinase-mediated cofilin-1 activity. Exp Ther Med 15:1269-1276 (2018). PubMed: 29434713