Overview

  • Product name
  • Description
    Rabbit polyclonal to Cofilin 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide:

    C-QVNGLDDIKDRSTLGEK

    conjugated to KLH, corresponding to amino acids 136-152 (plus N terminal cysteine) of Human Cofilin 2.

  • Positive control
    • Recombinant Human Cofilin 2 protein (ab114614) can be used as a positive control in WB. Rat L6 lysate.
  • General notes
    Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa.

Target

  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in disease
    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

References

This product has been referenced in:
  • Liang S  et al. Analysis of the protein complex associated with 14-3-3 epsilon by a deuterated-leucine labeling quantitative proteomics strategy. J Chromatogr B Analyt Technol Biomed Life Sci 877:627-34 (2009). Read more (PubMed: 19201265) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products. We regret to inform you that the cross-reactivity of ab11062 (cofilin 1) with cofilin 2 has not been tested yet. On the basis of poor sequence homology between human Cofilin 1 and Cofilin 2 for the sequence used as the immunogen for ab14133, it appears very unlikely that antibody will cross- react with human Cofilin 1. If you look at the large amount of homology throughout most of the two sequences until you get to aa 136, it is clear that this fragment was chosen to distinguish the two isoforms. Human Cofilin 2 1 masgvtvnde vikvfndmkv rksstqeeik krkkavlfcl sddkrqiive eakqilvgdi 61 gdtvedpyts fvkllplndc ryalydatye tkeskkedlv fifwapesap lkskmiyass 121 kdaikkkftg ikhewqvngl ddikdrstlg eklggnvvvs legkpl Human Cofilin 1 1 masgvavsdg vikvfndmkv rksstpeevk krkkavlfcl sedkkniile egkeilvgdv 61 gqtvddpyat fvkmlpdkdc ryalydatye tkeskkedlv fifwapesap lkskmiyass 121 kdaikkkltg ikhelqancy eevkdrctla eklggsavis legkpl We hope that you will find this information useful.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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