Key features and details
- Rabbit polyclonal to Cofilin 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cofilin 2 antibody
See all Cofilin 2 primary antibodies
DescriptionRabbit polyclonal to Cofilin 2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within amino acids 92 and 152 of Cofilin 2.
- WB: 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: gastric carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Tissue specificityIsoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
Involvement in diseaseDefects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
Sequence similaritiesBelongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.
modificationsThe phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton.
- Information by UniProt
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
Anti-Cofilin 2 antibody (ab96678) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS Page
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96678 at 1/200 dilution.
Immunohistochemistry analysis of paraffin-embedded gastric carcinoma, using ab96678 at 1/100 dilution.
ab96678 has been referenced in 3 publications.
- Wang J et al. Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation. Stem Cell Res Ther 10:349 (2019). PubMed: 31775893
- Yu BB et al. Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma. Med Sci Monit 24:2317-2329 (2018). PubMed: 29664897
- Chapnik E et al. miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. Elife 3:e01964 (2014). WB . PubMed: 24859754