Overview

  • Product name

  • Description

    Rabbit polyclonal to Cofilin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 92 and 152 of Cofilin 2.

  • Positive control

    • WB: 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: gastric carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab96678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity

    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in disease

    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similarities

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications

    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localization

    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Images

  • Anti-Cofilin 2 antibody (ab96678) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 18 kDa



    12% SDS Page
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96678 at 1/200 dilution.
  • Immunohistochemistry analysis of paraffin-embedded gastric carcinoma, using ab96678 at 1/100 dilution.

References

This product has been referenced in:

  • Yu BB  et al. Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma. Med Sci Monit 24:2317-2329 (2018). Read more (PubMed: 29664897) »
  • Chapnik E  et al. miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. Elife 3:e01964 (2014). WB . Read more (PubMed: 24859754) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

Abcam: {subject} Hi,   Thank you for your response. The antibody always gave a very good band result, but, the band do not have any difference between samples with and without CFL2-siRNA treatment. So, I am thinking whether this antibody has any crosslinking with CFL1, a protein which has much similarity to CFL2. Thanks for your patience.   Daya >>> 7/21/2011 6:54 PM >>> Dear Sir/Madam We have an answer to your inquiry: Thank you for your email. Yes, I've talked to Jeremy about this situation, too. Since the exact immunogen sequence is not known to me, but only to the lab, I could only do a BLAST search with the range of amino acids that was listed in our list. According to that, there was a pretty high percentage of similarity. However, it seems that when the lab BLASTed the actual sequence, there was no significant similarity found. The immunogen is probably in the region where there was the lowest amount of similarity. Therefore, I would go with the response that Jeremy sent you. My apologies for this confusion. I'm still a little bit confused by one of your previous responses. When I had sent you the protocol questions because you stated that you were seeing a band despite doing siRNA, you answered for the last question that "The antibody works well, and there is a very good and clear band there." Does this mean that you're still seeing the band in the siRNA-treated samples, or that the antibody is working fine now? My apologies for asking, but I'd just like to clarify. Also, do you have the order number for the purchase of this antibody? Thank you! Help us improve our service. Rate your experience with us today. Your original inquiry to Abcam:   I just got a letter from Jeremy. The letter is followed. It says that the antibody I am using will not be cross-link with CFL1, since no significant similarity was found by blasting the immunogen sequence.   Best regards, Hanwei Hanwei Li, PhD Scientific Support Specialist-California Abcam Inc. www.abcam.com Abcam Customer Services and Technical Support Team www.abcam.com/technical [CCE3002559] We have a HUGE selection of protocols and troubleshooting tips for IHC, WB, IP, ChIP, FACS, ELISA, ELISPOT and much more! www.abcam.com/protocols   On February 1st, MCG became Georgia Health Sciences University. My email address has also changed to the format "@georgiahealth.edu". Please update your address book to reflect this change.

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Answer

The immunogen is found within amino acids 92 to 152. I asked the lab to align the immunogen used to create ab96678 and the amino acid sequence of Cofilin 1. The percentage of mismatches between them is about 30%. At most there will be minimal cross reactivity of this antibody to Cofilin 1. I hope this is helpful. Please contact us again if you have any further questions.

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