Anti-Cofilin 2 antibody - C-terminal (ab179935)
Key features and details
- Goat polyclonal to Cofilin 2 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cofilin 2 antibody - C-terminal
See all Cofilin 2 primary antibodies -
Description
Goat polyclonal to Cofilin 2 - C-terminal -
Host species
Goat -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Cofilin 2 aa 142-152 (C terminal) (Cysteine residue).
Sequence:DIKDRSTLGEK
Database link: Q9Y281 -
Positive control
- Human muscle lysate; Human heart tissue.
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General notes
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
Horse
Chicken
Hamster
Cow
Dog
Pig
Monkey
Gorilla
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Application | Abreviews | Notes |
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
Tissue specificity
Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues. -
Involvement in disease
Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved. -
Sequence similarities
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
Post-translational
modificationsThe phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 539332 Cow
- Entrez Gene: 490649 Dog
- Entrez Gene: 100050494 Horse
- Entrez Gene: 1073 Human
- Entrez Gene: 12632 Mouse
- Entrez Gene: 497233 Pig
- Entrez Gene: 366624 Rat
- Omim: 601443 Human
see all -
Alternative names
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody - C-terminal (ab179935)
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human heart tissue labeling Cofilin 2 with ab179935 at 3.75 µg/ml.
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Anti-Cofilin 2 antibody - C-terminal (ab179935) at 0.03 µg/ml + Human muscle lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 1 hour
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab179935 has not yet been referenced specifically in any publications.