Anti-Cofilin 2 antibody - C-terminal (ab179935)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody - C-terminal (ab179935)
  • Western blot - Anti-Cofilin 2 antibody - C-terminal (ab179935)

Key features and details

  • Goat polyclonal to Cofilin 2 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cofilin 2 antibody - C-terminal
    See all Cofilin 2 primary antibodies

  • Description

    Goat polyclonal to Cofilin 2 - C-terminal

  • Host species

    Goat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Cofilin 2 aa 142-152 (C terminal) (Cysteine residue).
    Sequence:

    DIKDRSTLGEK


    Database link: Q9Y281
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human muscle lysate; Human heart tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Horse
Chicken
Hamster
Cow
Dog
Pig
Monkey
Gorilla
Application Abreviews Notes
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

  • Tissue specificity

    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

  • Involvement in disease

    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.

  • Sequence similarities

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.

  • Post-translational
    modifications

    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.

  • Cellular localization

    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody - C-terminal (ab179935)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody - C-terminal (ab179935)

    Immunohistochemical analysis of formalin-fixed, paraffin embedded Human heart tissue labeling Cofilin 2 with ab179935 at 3.75 µg/ml.

  • Western blot - Anti-Cofilin 2 antibody - C-terminal (ab179935)
    Western blot - Anti-Cofilin 2 antibody - C-terminal (ab179935)
    Anti-Cofilin 2 antibody - C-terminal (ab179935) at 0.03 µg/ml + Human muscle lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 19 kDa


    Exposure time: 1 hour

References (0)

Publishing research using ab179935? Please let us know so that we can cite the reference in this datasheet.

ab179935 has not yet been referenced specifically in any publications.

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