Anti-Cofilin 2 antibody - C-terminal (ab179935)
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- References
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Overview
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Product name
Anti-Cofilin 2 antibody - C-terminal
See all Cofilin 2 primary antibodies -
Description
Goat polyclonal to Cofilin 2 - C-terminal -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Cofilin 2 aa 142-152 (C terminal) (Cysteine residue).
Sequence:DIKDRSTLGEK
Database link: Q9Y281 -
Positive control
- Human muscle lysate; Human heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab179935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa. | |
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
Tissue specificity
Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues. -
Involvement in disease
Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved. -
Sequence similarities
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
Post-translational
modificationsThe phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 539332 Cow
- Entrez Gene: 490649 Dog
- Entrez Gene: 100050494 Horse
- Entrez Gene: 1073 Human
- Entrez Gene: 12632 Mouse
- Entrez Gene: 497233 Pig
- Entrez Gene: 366624 Rat
- Omim: 601443 Human
see all -
Alternative names
- CFL 2 antibody
- CFL2 antibody
- COF2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody - C-terminal (ab179935)Immunohistochemical analysis of formalin-fixed, paraffin embedded Human heart tissue labeling Cofilin 2 with ab179935 at 3.75 µg/ml.
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Western blot - Anti-Cofilin 2 antibody - C-terminal (ab179935)Anti-Cofilin 2 antibody - C-terminal (ab179935) at 0.03 µg/ml + Human muscle lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 1 hour
Datasheets and documents
References
ab179935 has not yet been referenced specifically in any publications.
