Overview

  • Product name

  • Description

    Rabbit polyclonal to Cofilin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cofilin (internal sequence). The exact sequence is proprietary.
    Database link: P23528

  • Positive control

    • WB: HEK-293T whole cell extract; A431, PC-12, Rat2 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. IHC-P: HBL435 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity

    Widely distributed in various tissues.
  • Sequence similarities

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-3 in resting cells.
  • Cellular localization

    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links

  • Alternative names

    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Images

  • All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : Cofilin shRNA-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/1000 dilution in ICC/IF. Untreated HeLa cells (left images) and HeLa cells treated with 0.012 N NaOH/PBS for 1 minute (right images). Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/1000 dilution.

  • Paraffin-embedded HBL435 xenograft tissue stained for Cofilin using ab227299 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Cofilin antibody (ab227299) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/500 dilution in ICC/IF. Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/2000 dilution.

  • Anti-Cofilin antibody (ab227299) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

References

ab227299 has not yet been referenced specifically in any publications.

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