Overview

  • Product name

    Anti-Cofilin antibody [EP6376]
    See all Cofilin primary antibodies
  • Description

    Rabbit monoclonal [EP6376] to Cofilin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cofilin (UniProt ID: P23528).

  • Positive control

    • WB: HeLa, MCF7, and Caco-2 cell lysates. IHC-P: Human colon tissue. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
    • Tissue specificity

      Widely distributed in various tissues.
    • Sequence similarities

      Belongs to the actin-binding proteins ADF family.
      Contains 1 ADF-H domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-3 in resting cells.
    • Cellular localization

      Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
    • Information by UniProt
    • Database links

    • Alternative names

      • 18 kDa phosphoprotein antibody
      • CFL 1 antibody
      • CFL antibody
      • CFL1 antibody
      • COF1_HUMAN antibody
      • Cofilin 1 antibody
      • Cofilin 1 non muscle antibody
      • Cofilin antibody
      • Cofilin non muscle isoform antibody
      • Cofilin-1 antibody
      • epididymis secretory protein Li 15 antibody
      • HEL-S-15 antibody
      • non-muscle isoform antibody
      • p18 antibody
      see all

    Images

    • All lanes : Anti-Cofilin antibody [EP6376] (ab134963) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Cofilin with ab134963 antibody at a dilution of 1/100.
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cofilin (red) with ab134963 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:

    • Cheng L  et al. Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity. Arch Toxicol 92:469-485 (2018). Read more (PubMed: 28871336) »
    • Chen WL  et al. Inhibition of TRPM7 by carvacrol suppresses glioblastoma cell proliferation, migration and invasion. Oncotarget 6:16321-40 (2015). Human . Read more (PubMed: 25965832) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134963.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up