Recombinant Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-243822-anti-cofilin-antibody-epr6375-loading-control-western-blot.jpg "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6375] to Cofilin - Loading Control
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cofilin antibody [EPR6375] - Loading Control
See all Cofilin primary antibodies -
Description
Rabbit monoclonal [EPR6375] to Cofilin - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cofilin aa 1-100. The exact sequence is proprietary.
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Positive control
- 293T and Caco-2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6375 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 19 kDa.
|
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| ICC/IF |
1/50 - 1/200.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 19 kDa. |
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ICC/IF
1/50 - 1/200.
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Target
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Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
Tissue specificity
Widely distributed in various tissues. -
Sequence similarities
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
Post-translational
modificationsPhosphorylated on Ser-3 in resting cells. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
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Database links
- Entrez Gene: 1072 Human
- Entrez Gene: 12631 Mouse
- Entrez Gene: 29271 Rat
- Omim: 601442 Human
- SwissProt: P23528 Human
- SwissProt: P18760 Mouse
- SwissProt: P45592 Rat
- Unigene: 170622 Human
see all -
Alternative names
- 18 kDa phosphoprotein antibody
- CFL 1 antibody
- CFL antibody
see all
Images
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Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : C6 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-268392-anti-cofilin-antibody-epr6375-loading-control-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
The nuclear counterstain is DAPI (blue).
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![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-243821-anti-cofilin-antibody-epr6375-loading-control-western-blot.jpg)
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified) + HEK293 cell lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-243820-anti-cofilin-antibody-epr6375-loading-control-western-blot.jpg)
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution (purified) + Caco-2 cell lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
-
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-139073-anti-cofilin-antibody-epr6375-loading-control-western-blot.jpg)
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa -
![Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)](https://www.abcam.com/ps/products/124/ab124979/Images/ab124979-9-benefits-of-recombinant-antibodies.png)
Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab124979 has been referenced in 8 publications.
- Zhang L et al. Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1. Front Cell Dev Biol 9:644716 (2021). PubMed: 33791303
- Jin L et al. ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis. Cancer Cell Int 20:589 (2020). PubMed: 33298070
- Yang W et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
- Zhang M et al. ZEB1-activated LINC01123 accelerates the malignancy in lung adenocarcinoma through NOTCH signaling pathway. Cell Death Dis 11:981 (2020). PubMed: 33191397
- Fei X et al. Eupatilin inhibits glioma proliferation, migration, and invasion by arresting cell cycle at G1/S phase and disrupting the cytoskeletal structure. Cancer Manag Res 11:4781-4796 (2019). PubMed: 31213900
- Kopp S et al. The role of NF?B in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine. Sci Rep 8:921 (2018). WB ; Human . PubMed: 29343717
- Sukma Dewi I et al. Exosomal miR-142-3p is increased during cardiac allograft rejection and augments vascular permeability through down-regulation of endothelial RAB11FIP2 expression. Cardiovasc Res 113:440-452 (2017). PubMed: 28073833
- Xu Z et al. Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism. Oncotarget 7:70559-70574 (2016). PubMed: 27708241