RecombinantRabMAb

Recombinant Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

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Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
  • Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6375] to Cofilin - Loading Control
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cofilin antibody [EPR6375] - Loading Control
    See all Cofilin primary antibodies

  • Description

    Rabbit monoclonal [EPR6375] to Cofilin - Loading Control

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cofilin aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T and Caco-2 cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 19 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.
ICC/IF
1/50 - 1/200.

 
Notes
WB
1/1000. Predicted molecular weight: 19 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.
ICC/IF
1/50 - 1/200.

 

Target

  • Function

    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

  • Tissue specificity

    Widely distributed in various tissues.

  • Sequence similarities

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.

  • Post-translational
    modifications

    Phosphorylated on Ser-3 in resting cells.

  • Cellular localization

    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Images

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified)

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : C6 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

    Immunohistochemical staining of paraffin embedded mouse liver with purified ab124979 at a working dilution of 1 in 200. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    The nuclear counterstain is DAPI (blue).

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified) + HEK293 cell lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution (purified) + Caco-2 cell lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab124979 at a working dilution of 1 in 200. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 19 kDa

  • Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
    Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

References (5)

Publishing research using ab124979? Please let us know so that we can cite the reference in this datasheet.

ab124979 has been referenced in 5 publications.

  • Yang W  et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
  • Fei X  et al. Eupatilin inhibits glioma proliferation, migration, and invasion by arresting cell cycle at G1/S phase and disrupting the cytoskeletal structure. Cancer Manag Res 11:4781-4796 (2019). PubMed: 31213900
  • Kopp S  et al. The role of NF?B in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine. Sci Rep 8:921 (2018). WB ; Human . PubMed: 29343717
  • Sukma Dewi I  et al. Exosomal miR-142-3p is increased during cardiac allograft rejection and augments vascular permeability through down-regulation of endothelial RAB11FIP2 expression. Cardiovasc Res 113:440-452 (2017). PubMed: 28073833
  • Xu Z  et al. Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism. Oncotarget 7:70559-70574 (2016). PubMed: 27708241

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