Key features and details
- Rabbit polyclonal to COG4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Product nameAnti-COG4 antibody
See all COG4 primary antibodies
DescriptionRabbit polyclonal to COG4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
Recombinant fragment corresponding to Human COG4 aa 506-785.
FQDIQRGVTSAVNIMHSSLQQGKFDTKGIESTDEAKMSFLVTLNNVEVCS ENISTLKKTLESDCTKLFSQGIGGEQAQAKFDSCLSDLAAVSNKFRDLLQ EGLTELNSTAIKPQVQPWINSFFSVSHNIEEEEFNDYEANDPWVQQFILN LEQQMAEFKASLSPVIYDSLTGLMTSLVAVELEKVVLKSTFNRLGGLQFD KELRSLIAYLTTVTTWTIRDKFARLSQMATILNLERVTEILDYWGPNSGP LTWRLTPAEVRQVLALRIDFRSEDIKRLRL
Database link: Q9H9E3
- WB: Mouse liver and gonadal tissue lysates; K562 and HeLa whole cell lysates. IHC-P: Human tonsil and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 89 kDa.|
RelevanceCOG4 is a component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. COG4 is required for normal Golgi function.
Cellular localizationGolgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
- CDG2J antibody
- COD1 antibody
- COG 4 antibody
All lanes : Anti-COG4 antibody (ab229953) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse gonadal tissue lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 89 kDa
Observed band size: 89 kDa
Paraffin-embedded human tonsil tissue stained for COG4 using ab229953 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for COG4 using ab229953 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229953 has not yet been referenced specifically in any publications.