Product nameAnti-COG6 antibody - C-terminal
See all COG6 primary antibodies
DescriptionRabbit polyclonal to COG6 - C-terminal
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Chinese hamster
Synthetic peptide within Human COG6 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. The corresponding sequence differs by three amino acids in mouse and rat COG6.
Database link: Q9Y2V7
- HEK293T cell lysate overexpressing Human COG6; HEK293T, HeLa, A341, HepG2, LA4, PC12 and CHO cell lysates; Mouse testis tissue lysate; HeLa cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.098% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab214062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 5 - 10 μg per reaction.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 73 kDa.|
|ICC/IF||Use a concentration of 2 µg/ml.|
FunctionRequired for normal Golgi function.
Sequence similaritiesBelongs to the COG6 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- CDG2L antibody
- COD2 antibody
- COG complex subunit 6 antibody
Immunofluorescent analysis of paraformaldehyde (1% in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl2 and 2.5% glycerol) fixed and 0.1% Triton X-100 permeabilized HeLa cells, labeling COG6 with ab214062 at 2 µg/mL, followed by Goat Anti-Rabbit IgG Cy3 conjugate (red, panel A and B) and Golgi marker labeling (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B).
All lanes : Anti-COG6 antibody - C-terminal (ab214062) at 4 µg/ml
Lane 1 : HEK293T cell lysate overexpressing Human COG6
Lane 2 : HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A431 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : LA4 cell lysate
Lane 7 : Mouse testis tissue lysate
Lane 8 : PC12 cell lysate
Lane 9 : CHO cell lysate
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 73 kDa
ab214062 was used to immunoprecipitate COG6 from HeLa whole cell extract.
1. 5 μg ab214062
2. 10 μg ab214062
3. Negative control: 5 μg non-relevant antibody
ab214062 has not yet been referenced specifically in any publications.