Overview

  • Product name

    Anti-COG6 antibody - C-terminal
    See all COG6 primary antibodies
  • Description

    Rabbit polyclonal to COG6 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Chinese hamster
  • Immunogen

    Synthetic peptide within Human COG6 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. The corresponding sequence differs by three amino acids in mouse and rat COG6.
    Database link: Q9Y2V7

  • Positive control

    • HEK293T cell lysate overexpressing Human COG6; HEK293T, HeLa, A341, HepG2, LA4, PC12 and CHO cell lysates; Mouse testis tissue lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab214062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 5 - 10 μg per reaction.

WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 2 µg/ml.

Target

Images

  • Immunofluorescent analysis of paraformaldehyde (1% in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl2 and 2.5% glycerol) fixed and 0.1% Triton X-100 permeabilized HeLa cells, labeling COG6 with ab214062 at 2 µg/mL, followed by Goat Anti-Rabbit IgG Cy3 conjugate (red, panel A and B) and Golgi marker labeling (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B).

  • All lanes : Anti-COG6 antibody - C-terminal (ab214062) at 4 µg/ml

    Lane 1 : HEK293T cell lysate overexpressing Human COG6
    Lane 2 : HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : LA4 cell lysate
    Lane 7 : Mouse testis tissue lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : CHO cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • ab214062 was used to immunoprecipitate COG6 from HeLa whole cell extract. 
    Lanes
    1. 5 μg ab214062
    2. 10 μg ab214062
    3. Negative control: 5 μg non-relevant antibody

References

ab214062 has not yet been referenced specifically in any publications.

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