Key features and details
- Rabbit polyclonal to COG7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COG7 antibody
See all COG7 primary antibodies
DescriptionRabbit polyclonal to COG7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human COG7 aa 720-770. The exact sequence is proprietary.
Database link: P83436
- IP: HeLa whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226366 was affinity purified using an epitope specific to COG7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for normal Golgi function.
Involvement in diseaseDefects in COG7 are the cause of congenital disorder of glycosylation type 2E (CDG2E) [MIM:608779]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
Sequence similaritiesBelongs to the COG7 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- CDG2E antibody
- COG complex subunit 7 antibody
- cog7 antibody
All lanes : Anti-COG7 antibody (ab226366) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes
Cell lysates were prepared with NETN lysis buffer.
COG7 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226366 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226366 at 1 µg/ml.
Lane 1: ab226366 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226366 has not yet been referenced specifically in any publications.