Overview

  • Product name

  • Description

    Rabbit polyclonal to COG7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human COG7 aa 720-770. The exact sequence is proprietary.
    Database link: P83436

  • Positive control

    • IP: HeLa whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226366 was affinity purified using an epitope specific to COG7 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Required for normal Golgi function.
  • Involvement in disease

    Defects in COG7 are the cause of congenital disorder of glycosylation type 2E (CDG2E) [MIM:608779]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • Sequence similarities

    Belongs to the COG7 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDG2E antibody
    • COG complex subunit 7 antibody
    • cog7 antibody
    • COG7_HUMAN antibody
    • Component of oligomeric Golgi complex 7 antibody
    • Conserved oligomeric Golgi complex component 7 antibody
    • Conserved oligomeric Golgi complex subunit 7 antibody
    see all

Images

  • All lanes : Anti-COG7 antibody (ab226366) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 86 kDa


    Exposure time: 3 minutes


    Cell lysates were prepared with NETN lysis buffer.

  • COG7 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226366 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226366 at 1 µg/ml.

    Lane 1: ab226366 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226366 has not yet been referenced specifically in any publications.

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