Recombinant Anti-COG7 antibody [EPR9942] (ab168362)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9942] to COG7
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-COG7 antibody [EPR9942]
See all COG7 primary antibodies -
Description
Rabbit monoclonal [EPR9942] to COG7 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human COG7.
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Positive control
- MOLT4, HeLa, MCF7, C6, RAW 264.7, PC12, and NIH 3T3 cell lysates; Human brain and kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9942 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
|
ICC/IF |
1/100 - 1/250.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 86 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Required for normal Golgi function. -
Involvement in disease
Defects in COG7 are the cause of congenital disorder of glycosylation type 2E (CDG2E) [MIM:608779]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. -
Sequence similarities
Belongs to the COG7 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 91949 Human
- Entrez Gene: 233824 Mouse
- Entrez Gene: 293456 Rat
- Omim: 606978 Human
- SwissProt: P83436 Human
- SwissProt: Q3UM29 Mouse
- SwissProt: Q3T1G7 Rat
- Unigene: 185807 Human
see all -
Alternative names
- CDG2E antibody
- COG complex subunit 7 antibody
- cog7 antibody
see all
Images
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All lanes : Anti-COG7 antibody [EPR9942] (ab168362) at 1/1000 dilution
Lane 1 : MOlT4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COG7 with ab168362 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG7 with ab168362 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling COG7 with ab168362 at 1/100 dilution. DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling COG7 (red) with ab168362 at 1/10 dilution, compared to a nonspecific control antibody (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab168362 has been referenced in 1 publication.
- Liang J et al. Verteporfin Inhibits PD-L1 through Autophagy and the STAT1-IRF1-TRIM28 Signaling Axis, Exerting Antitumor Efficacy. Cancer Immunol Res 8:952-965 (2020). PubMed: 32265228