Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-Coilin antibody [EPR7843(N)] - BSA and Azide free
See all Coilin primary antibodies
DescriptionRabbit monoclonal [EPR7843(N)] to Coilin - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P38432
- WB: Jurkat and HeLa cell lysates.
ab249972 is the carrier-free version of ab177466 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab249972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.|
FunctionIs a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
Tissue specificityFound in all the cell types examined.
Sequence similaritiesBelongs to the coilin family.
Cellular localizationNucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
- Information by UniProt
- CLN 80 antibody
- CLN80 antibody
- COIL antibody
All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COIL knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab177466).
ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249972 has not yet been referenced specifically in any publications.