Recombinant Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Coilin antibody [EPR7843(N)] - N-terminal
See all Coilin primary antibodies -
Description
Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P38432 -
Positive control
- WB: Jurkat, HeLa, MCF7 and HepG2 cell lysates. Flow Cyt (intra): Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7843(N) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
|
|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa. |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. -
Tissue specificity
Found in all the cell types examined. -
Sequence similarities
Belongs to the coilin family. -
Cellular localization
Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8161 Human
- Omim: 600272 Human
- SwissProt: P38432 Human
- Unigene: 532795 Human
-
Alternative names
- CLN 80 antibody
- CLN80 antibody
- COIL antibody
see all
Images
-
All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COIL knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab177466 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green).
-
All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab177466 has not yet been referenced specifically in any publications.