Recombinant
RabMAb

Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)

Overview

  • Product name
    Anti-Coilin antibody [EPR7843(N)] - N-terminal
    See all Coilin primary antibodies
  • Description
    Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P38432

  • Positive control
    • Jurkat, HeLa, MCF7 and HepG2 cell lysates. Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
    • Tissue specificity
      Found in all the cell types examined.
    • Sequence similarities
      Belongs to the coilin family.
    • Cellular localization
      Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CLN 80 antibody
      • CLN80 antibody
      • COIL antibody
      • COIL_HUMAN antibody
      • Coilin antibody
      • Coilin p80 antibody
      • p80 antibody
      • P80 coilin antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green).

    • All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 63 kDa

    References

    ab177466 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177466.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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