Product nameAnti-Coilin antibody [EPR7843(N)] - N-terminal
See all Coilin primary antibodies
DescriptionRabbit monoclonal [EPR7843(N)] to Coilin - N-terminal
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P38432
- Jurkat, HeLa, MCF7 and HepG2 cell lysates. Jurkat cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 63 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIs a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
Tissue specificityFound in all the cell types examined.
Sequence similaritiesBelongs to the coilin family.
Cellular localizationNucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
- Information by UniProt
- CLN 80 antibody
- CLN80 antibody
- COIL antibody
Flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green).
All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
ab177466 has not yet been referenced specifically in any publications.