Anti-Coilin antibody [IH10] (ab87913)
![Western blot - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-384496-anti-coilin-antibody-ih10-western-blot-hela-jurkat.jpg "Western blot - Anti-Coilin antibody [IH10] (ab87913)")
Key features and details
- Mouse monoclonal [IH10] to Coilin
- Suitable for: IP, IHC-P, Flow Cyt, WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Coilin antibody [IH10]
See all Coilin primary antibodies -
Description
Mouse monoclonal [IH10] to Coilin -
Host species
Mouse -
Tested applications
Suitable for: IP, IHC-P, Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Coilin.
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Positive control
- WB: HeLa, Jurkat, HepG2, Hek293, MCF7, Caco-2 and SHSY-5Y cell lysates. IHC-P: Human normal testis. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
IH10 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
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| ICC/IF | (1) |
Use a concentration of 5 - 10 µg/ml.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 5 µg/ml. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa). |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
Target
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Function
Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. -
Tissue specificity
Found in all the cell types examined. -
Sequence similarities
Belongs to the coilin family. -
Cellular localization
Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8161 Human
- Omim: 600272 Human
- SwissProt: P38432 Human
- Unigene: 532795 Human
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Alternative names
- CLN 80 antibody
- CLN80 antibody
- COIL antibody
see all
Images
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Western blot - Anti-Coilin antibody [IH10] (ab87913)All lanes : Anti-Coilin antibody [IH10] (ab87913) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COIL knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab87913 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab87913 Anti-Coilin antibody [IH10] was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab87913 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102811-anti-coilin-antibody-ih10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [IH10] (ab87913)This image is part of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab87913(1/2000) staining Coilin in assynchronous HeLa cells (green). Cells were fixed in methanol (this image) or paraformaldehyde (see abreview image), permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-226232-anti-coilin-antibody-ih10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coilin antibody [IH10] (ab87913)IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab87913, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102810-anti-coilin-antibody-ih10-western-blot.jpg)
Western blot - Anti-Coilin antibody [IH10] (ab87913)All lanes : Anti-Coilin antibody [IH10] (ab87913) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 8 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
![Flow Cytometry - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102809-anti-coilin-antibody-ih10-flow-cytometry.jpg)
Flow Cytometry - Anti-Coilin antibody [IH10] (ab87913)Overlay histogram showing HeLa cells stained with ab87913 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87913, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102808-anti-coilin-antibody-ih10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coilin antibody [IH10] (ab87913)IHC image of Coilin staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87913, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunoprecipitation - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102807-anti-coilin-antibody-ih10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Coilin antibody [IH10] (ab87913)Coilin was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to Coilin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab87913.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 75kDa: Coilin -
![Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [IH10] (ab87913)](https://www.abcam.com/ps/products/87/ab87913/Images/ab87913-102806-anti-coilin-antibody-ih10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [IH10] (ab87913)ICC/IF image of ab87913 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87913, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (24)
ab87913 has been referenced in 24 publications.
- Xu C et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death Differ 27:1998-2013 (2020). PubMed: 31863069
- Ding H et al. Systematic Analysis of Drug Vulnerabilities Conferred by Tumor Suppressor Loss. Cell Rep 27:3331-3344.e6 (2019). PubMed: 31189115
- Jackson MR et al. Radiolabeled Oligonucleotides Targeting the RNA Subunit of Telomerase Inhibit Telomerase and Induce DNA Damage in Telomerase-Positive Cancer Cells. Cancer Res 79:4627-4637 (2019). PubMed: 31311806
- Deng T et al. TOE1 acts as a 3' exonuclease for telomerase RNA and regulates telomere maintenance. Nucleic Acids Res 47:391-405 (2019). PubMed: 30371886
- Ho ST et al. The PinX1/NPM interaction associates with hTERT in early-S phase and facilitates telomerase activation. Cell Biosci 9:47 (2019). PubMed: 31210926
