Overview

  • Product name

    Anti-Coilin antibody [IH10] (HRP)
    See all Coilin primary antibodies
  • Description

    Mouse monoclonal [IH10] to Coilin (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Coilin.

  • Positive control

    • WB: HeLa, MCF7 and SHSY-5Y whole cell lysates. IHC-P: FFPE Human Testis Normal
  • General notes

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab196715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
  • Tissue specificity

    Found in all the cell types examined.
  • Sequence similarities

    Belongs to the coilin family.
  • Cellular localization

    Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLN 80 antibody
    • CLN80 antibody
    • COIL antibody
    • COIL_HUMAN antibody
    • Coilin antibody
    • Coilin p80 antibody
    • p80 antibody
    • P80 coilin antibody
    see all

Images

  • IHC image of Collin staining in a section of formalin-fixed paraffin-embedded normal human testis*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab196715 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. 

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Coilin antibody [IH10] (HRP) (ab196715) at 1/2000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196715 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab196715 has not yet been referenced specifically in any publications.

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