• Product name
    Anti-Coilin antibody [Pdelta]
    See all Coilin primary antibodies
  • Description
    Mouse monoclonal [Pdelta] to Coilin
  • Host species
  • Tested applications
    Suitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human Coilin (C terminal).

  • Epitope
    Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.
  • Positive control
    • HeLa nuclear extract.



Our Abpromise guarantee covers the use of ab11822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa.
ICC/IF Use a concentration of 1 µg/ml.


  • Function
    Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
  • Tissue specificity
    Found in all the cell types examined.
  • Sequence similarities
    Belongs to the coilin family.
  • Cellular localization
    Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLN 80 antibody
    • CLN80 antibody
    • COIL antibody
    • COIL_HUMAN antibody
    • Coilin antibody
    • Coilin p80 antibody
    • p80 antibody
    • P80 coilin antibody
    see all


  • ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Lin Y  et al. Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers. Cell 167:789-802.e12 (2016). Human . Read more (PubMed: 27768897) »
  • Lee JH  et al. Involvement of SRSF11 in cell cycle-specific recruitment of telomerase to telomeres at nuclear speckles. Nucleic Acids Res 43:8435-51 (2015). ICC/IF ; Human . Read more (PubMed: 26286192) »
See all 29 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (H1299)
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 12 2010


Thank you for your enquiry. We would like to confirm that this antibody has been tested and characterized for Western blot and ICC application. It recognizes human Coilin but it does not cross-react with Mouse However we would like to emphasize the following: For immunohistochemistry, the primary antibody should be raised in a species as phylogenetically different as possible to the species that your sample was obtained. This is to avoid the secondary antibody cross-reacting with endogenous immunoglobulins in the sample, leading to erroneous results. For instance, this primary antibody used to detect a protein in a sample from a mouse should not be raised in mouse or rat. A primary antibody raised in rabbit would be a more appropriate choice. This is not really an issue with other immunological techniques such as Western blot application where samples do not contain endogenous immunoglobulin. For further information, please take a look at the following section of our Antibody Guide module on HOW TO CHOOSE AN ANTIBODY https://www.abcam.com/index.html?pageconfig=tech&mode=choosing If you need anything further or any help then please let us know.

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