Overview

  • Product name

  • Description

    Rabbit polyclonal to COL11A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from an internal sequence of Human COL11A1.

  • Positive control

    • K562 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/50. (see Abreview)
WB 1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 181 kDa).
ELISA 1/10000.

Target

  • Function

    May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
  • Tissue specificity

    Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
  • Involvement in disease

    Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable.
    Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • COBA1_HUMAN antibody
    • COL11A1 antibody
    • COLL6 antibody
    • Collagen alpha 1 antibody
    • Collagen alpha-1(XI) chain antibody
    • collagen XI alpha 1 antibody
    • collagen XI, alpha 1 polypeptide antibody
    • collagen, type XI, alpha 1 antibody
    • STL2 antibody
    • STL3 antibody
    • XI chain precursor antibody
    see all

Images

  • All lanes : Anti-COL11A1 antibody (ab64883) at 1/500 dilution

    Lane 1 : extracts from K562 cells (5-30µg total protein)
    Lane 2 : extracts from K562 cells (5-30µg total protein) and 5-10µg of the immunising peptide.

    Predicted band size: 181 kDa
    Observed band size: 181 kDa

  • ab64883 staining of frozen human fetal heart tissue sections (IHC-Fr). Sections were fixed with paraformaldehyde and permeabilized by saponin. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour 30 minutes at 37°C. An Alexa Fluor conjugated goat anti-rabbit antibody was used as the secondary.

    This image is courtesy of an anonymous Abreview (April 2009)

  • Immunohistochemical analysis of frozen murine ovary tissue taken from post-natal day 23-29 wild-type (+/+) and ERß-null (-/-) mice.

    COL11A1 was stained with ab64883, at 1/200 dilution.

References

This product has been referenced in:

  • Zheng C  et al. Suppressing UPR-dependent overactivation of FGFR3 signaling ameliorates SLC26A2-deficient chondrodysplasias. EBioMedicine 40:695-709 (2019). Read more (PubMed: 30685387) »
  • Wang Y & Wu X SMOC1 silencing suppresses the angiotensin II-induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway. Oncol Lett 16:2903-2910 (2018). Read more (PubMed: 30127878) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 11 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF, R1 ES cells, E14 ES cells)
Specification
MEF, R1 ES cells, E14 ES cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton

Abcam user community

Verified customer

Submitted Jun 20 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human fetal heart)
Specification
human fetal heart
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% saponin in PBS
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 06 2009

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