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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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RecombinantRabMAb

Recombinant Anti-COL11A1 antibody [EPR8294] (ab166606)

  • Datasheet
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Western blot - Anti-COL11A1 antibody [EPR8294] (ab166606)
  • Anti-COL11A1 antibody [EPR8294] (ab166606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8294] to COL11A1
  • Suitable for: WB
  • Reacts with: Human

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Product image
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Overview

  • Product name

    Anti-COL11A1 antibody [EPR8294]
    See all COL11A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8294] to COL11A1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human COL11A1 (UniProt ID: P12107).

  • Positive control

    • JAR, Saos-2 and K562 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8294
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen

Associated products

  • Alternative Versions

    • Anti-COL11A1 antibody [EPR8294] - BSA and Azide free (ab249347)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 181 kDa).
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 181 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
    • Tissue specificity

      Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
    • Involvement in disease

      Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable.
      Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.
    • Sequence similarities

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 TSP N-terminal (TSPN) domain.
    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P12107 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1301 Human
      • Omim: 120280 Human
      • SwissProt: P12107 Human
      • Unigene: 523446 Human
      • Alternative names

        • COBA1_HUMAN antibody
        • COL11A1 antibody
        • COLL6 antibody
        • Collagen alpha 1 antibody
        • Collagen alpha-1(XI) chain antibody
        • collagen XI alpha 1 antibody
        • collagen XI, alpha 1 polypeptide antibody
        • collagen, type XI, alpha 1 antibody
        • STL2 antibody
        • STL3 antibody
        • XI chain precursor antibody
        see all

      Images

      • Western blot - Anti-COL11A1 antibody [EPR8294] (ab166606)
        Western blot - Anti-COL11A1 antibody [EPR8294] (ab166606)
        All lanes : Anti-COL11A1 antibody [EPR8294] (ab166606) at 1/1000 dilution

        Lane 1 : JAR cell lysate
        Lane 2 : Saos-2 cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 181 kDa
        Observed band size: 150 kDa
        why is the actual band size different from the predicted?

      • Anti-COL11A1 antibody [EPR8294] (ab166606)
        Anti-COL11A1 antibody [EPR8294] (ab166606)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab166606? Please let us know so that we can cite the reference in this datasheet.

      ab166606 has been referenced in 2 publications.

      • Liu Q  et al. PDGF-BB promotes the differentiation and proliferation of MC3T3-E1 cells through the Src/JAK2 signaling pathway. Mol Med Rep 18:3719-3726 (2018). PubMed: 30106097
      • Simon EP  et al. Transcriptomic characterization of fibrolamellar hepatocellular carcinoma. Proc Natl Acad Sci U S A 112:E5916-25 (2015). PubMed: 26489647

      Customer reviews and Q&As

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