Overview

  • Product name

  • Description

    Rabbit polyclonal to COL12A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 768-892 of Human COL12A1.

  • Positive control

    • Human skeletal muscle tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 333 kDa.

Target

  • Relevance

    Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • BA209D8.1 antibody
    • COCA1 antibody
    • COL12A1 antibody
    • COL12A1L antibody
    • Collagen alpha-1(XII) chain antibody
    • collagen type XII proteoglycan antibody
    • collagen, type XII, alpha 1 antibody
    • DJ234P15.1 antibody
    see all

Images

  • All lanes :

    Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
    Lane 2 : Human cell line U-2 OS

    Predicted band size: 333 kDa

  • ab121304 staining COL12A1 in Paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Zhang S  et al. hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC. Oncotarget 8:104508-104524 (2017). Read more (PubMed: 29262657) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse skeletal muscle)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Mouse skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 18 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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