Key features and details
- Rabbit polyclonal to COL16A1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COL16A1 antibody - C-terminal
DescriptionRabbit polyclonal to COL16A1 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human COL16A1 aa 1434-1604 (C terminal).
VNYDEIKRFIRQEIIKMFDERMAYYTSRMQFPMEMAAAPGRPGPPGKDGA PGRPGAPGSPGLPGQIGREGRQGLPGVRGLPGTKGEKGDIGIGIAGENGL PGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDC FGAMPMEQQYPPMKTMKGPFG
Database link: Q07092-1
- WB: Recombinant human COL16A1 protein; Human serum. IHC-P: Human lung tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231044 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 158 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionInvolved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology.
Tissue specificityIn papillary dermis, is a component of specialized fibrillin-1-containing microfibrils, whereas in territorial cartilage matrix, it is localized to a discrete population of thin, weakly banded collagen fibrils in association with other collagens (at protein level). In the placenta, where it is found in the amnion, a membranous tissue lining the amniotic cavity. Within the amnion, it is found in an acellular, relatively dense layer of a complex network of reticular fibers. Also located to a fibroblast layer beneath this dense layer. Exists in tissues in association with other types of collagen.
Sequence similaritiesBelongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Contains 1 TSP N-terminal (TSPN) domain.
Developmental stageTransiently elevated expression during gestation, and decrease at term.
DomainThis sequence defines eighteen different domains, nine triple-helical domains (COL9 to COL1) and ten non-triple-helical domains (NC10 to NC1). The numerous interruptions in the triple helix may make this molecule either elastic or flexible.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 447AA antibody
- Alpha 1 type XVI collagen antibody
- COGA1_HUMAN antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for COL16A1 with ab231044 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-COL16A1 antibody - C-terminal (ab231044) at 3 µg/ml + human serum
Predicted band size: 158 kDa
Anti-COL16A1 antibody - C-terminal (ab231044) at 2 µg/ml + recombinant human COL16A1 protein
Predicted band size: 158 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231044 has not yet been referenced specifically in any publications.