Anti-COL1A2 antibody (ab96723)

Reviews (4)Q&A (4)References (16)

Overview

  • Product name

    Anti-COL1A2 antibody

  • Description

    Rabbit polyclonal to COL1A2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Monkey

  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).

  • Positive control

    • WB: 293T and A431 cell lysate ICC/IF: A431 cell

Properties

Applications

Our Abpromise guarantee covers the use of ab96723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 129 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).

  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

  • Involvement in disease

    Ehlers-Danlos syndrome 7B
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.

  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.

  • Domain

    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha 2 collagen type I antibody
    • Alpha 2 type I collagen antibody
    • Alpha 2 type I procollagen antibody
    • Alpha 2(I) collagen antibody
    • Alpha 2(I) procollagen antibody
    • Alpha-2 type I collagen antibody
    • CO1A2_HUMAN antibody
    • COL1A2 antibody
    • Collagen alpha 2(I) chain antibody
    • Collagen alpha-2(I) chain antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen of skin tendon and bone alpha 2 chain antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis imperfecta type IV antibody
    • Type I procollagen antibody
    see all

Images

  • Western blot - Anti-COL1A2 antibody (ab96723)
    Western blot - Anti-COL1A2 antibody (ab96723)
    All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 129 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)
    Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)
    Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
    Lower image: merged with DNA probe.

References

This product has been referenced in:

  • Li J  et al. TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling. Nat Commun 10:1589 (2019). Read more (PubMed: 30962442) »
  • Yang F  et al. The Akt/FoxO/p27Kip1 axis contributes to the anti-proliferation of pentoxifylline in hypertrophic scars. J Cell Mol Med 23:6164-6172 (2019). Read more (PubMed: 31270945) »
See all 16 Publications for this product

Publishing research using ab96723? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-COL1A2 antibody

 Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Jan 23 2019

Western blot abreview for Anti-COL1A2 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Tissue lysate - other (Follicular fluids from ovarian follicles)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
35 µg
Specification
Follicular fluids from ovarian follicles
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 18 2015

Western blot abreview for Anti-COL1A2 antibody

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (prostate)
Specification
prostate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Xu Cheng

Verified customer

Submitted Sep 08 2014

Western blot abreview for Anti-COL1A2 antibody

 Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary calvarial cells)
Loading amount
20 µg
Specification
Primary calvarial cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Erica Perryn

Verified customer

Submitted Dec 20 2012

Question

I would like to test ab96723 for use on rat samples.

Read More
Answer

DISCOUNT CODE: ***
Expiration date: January 18th, 2013

I am very pleased to hear you would like to accept our offer and test ab96723 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Question

I recently was able to successfully test this antibody in mouse samples with positive results. I see my discount has expired. I would like to have it re-instated and I will provide you with results today on using the antibody with mouse cells.

Read More
Answer

Thank you for contacting us.

Yes, that is no problem and we are looking forward to receiving your Abreview. I am sending you another discount code for testing ab96723 in mouse, but please ensure that you use it before it expires, as extending a discount code is a one-time exception.


DISCOUNT CODE: xxx
Expiration date: xxx

I wish you good luck with your research!
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

WB conditions (reducing/non-reducing; denaturing/non-denaturing)? will test in mouse

Read More
Answer

Thank you for your patience. Yes, the lab confirmed that denaturing conditions have been used: The proteins were extracted in a RIPA buffer containing SDS. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Question

WB conditions (reducing/non-reducing; denaturing/non-denaturing)? will test in mouse

Read More
Answer

Thank you for your patience. I received the following information from the lab: The samples were boiled (at 100 degree C for 10 mins) under reducing condition. I assume this means also under denaturing conditions, but I am checking with them to confirm this. I will be in touch as soon as I find out. I hope this information is so far helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com