- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-COL1A2 antibody
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DescriptionRabbit polyclonal to COL1A2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Monkey
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).
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Positive control
- WB: 293T and A431 cell lysate ICC/IF: A431 cell
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab96723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 129 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
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Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
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Involvement in diseaseEhlers-Danlos syndrome 7B
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. -
Sequence similaritiesBelongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain. -
DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
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Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 282188 Cow
- Entrez Gene: 403824 Dog
- Entrez Gene: 1278 Human
- Entrez Gene: 12843 Mouse
- Entrez Gene: 84352 Rat
- Omim: 120160 Human
- SwissProt: P02465 Cow
- SwissProt: O46392 Dog
see all -
Alternative names
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2 type I procollagen antibody
see all
Images
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Western blot - Anti-COL1A2 antibody (ab96723)All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 129 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
Lower image: merged with DNA probe.
References
This product has been referenced in:
- Ruibin W et al. Micro RNA-1298 opposes the effects of chronic oxidative stress on human trabecular meshwork cells via targeting on EIF4E3. Biomed Pharmacother 100:349-357 (2018). WB . Read more (PubMed: 29453044) »
- Zhao B et al. Hypoxia drives the transition of human dermal fibroblasts to a myofibroblast-like phenotype via the TGF-ß1/Smad3 pathway. Int J Mol Med 39:153-159 (2017). WB ; Human . Read more (PubMed: 27909731) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"