Anti-COL1A2 antibody (ab96723)
Key features and details
- Rabbit polyclonal to COL1A2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COL1A2 antibody
See all COL1A2 primary antibodies -
Description
Rabbit polyclonal to COL1A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Monkey
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).
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Positive control
- WB: 293T and A431 cell lysate ICC/IF: A431 cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/500 - 1/3000. Predicted molecular weight: 129 kDa.
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| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 129 kDa. |
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ICC/IF
1/100 - 1/200. |
Target
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Ehlers-Danlos syndrome 7B
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 282188 Cow
- Entrez Gene: 403824 Dog
- Entrez Gene: 1278 Human
- Entrez Gene: 12843 Mouse
- Omim: 120160 Human
- SwissProt: P02465 Cow
- SwissProt: O46392 Dog
- SwissProt: P08123 Human
see all -
Alternative names
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2 type I procollagen antibody
see all
Images
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Western blot - Anti-COL1A2 antibody (ab96723)All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 129 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
Lower image: merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab96723 has been referenced in 25 publications.
- Rong X et al. Antler stem cells as a novel stem cell source for reducing liver fibrosis. Cell Tissue Res 379:195-206 (2020). PubMed: 31428875
- Li L et al. Human ß-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis. Int J Oral Sci 12:13 (2020). PubMed: 32350241
- Yang Y et al. ING4 alleviated lipopolysaccharide-induced inflammation by regulating the NF-?B pathway via a direct interaction with SIRT1. Immunol Cell Biol 98:127-137 (2020). PubMed: 31811786
- Jiang X et al. Low-Dose Aspirin Treatment Attenuates Male Rat Salt-Sensitive Hypertension via Platelet Cyclooxygenase 1 and Complement Cascade Pathway. J Am Heart Assoc 9:e013470 (2020). PubMed: 31852420
- Chen H et al. Thalidomide Prevented and Ameliorated Pathogenesis of Crohn's Disease in Mice via Regulation of Inflammatory Response and Fibrosis. Front Pharmacol 10:1486 (2019). PubMed: 31920668