Anti-COL4A2 antibody (ab125208)
Key features and details
- Rabbit polyclonal to COL4A2
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-COL4A2 antibody -
Description
Rabbit polyclonal to COL4A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human COL4A2 (C terminal).
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General notes
This product was previously labelled as CO4A2
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 168 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC |
Use a concentration of 2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 168 kDa. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC
Use a concentration of 2 µg/ml. |
Target
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Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. -
Sequence similarities
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
Domain
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, canstatin. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 1284 Human
- Entrez Gene: 12827 Mouse
- Entrez Gene: 306628 Rat
- Omim: 120090 Human
- SwissProt: P08572 Human
- SwissProt: P08122 Mouse
- Unigene: 508716 Human
- Unigene: 181021 Mouse
see all -
Alternative names
- Canstatin antibody
- CO4A2_HUMAN antibody
- COL4A 2 antibody
see all
Images
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Western blot - Anti-COL4A2 antibody (ab125208)All lanes :
Lane 1 : Rat Kidney Lysate
Lane 2 : Rat Lung Lysate
Predicted band size: 168 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat Kidney tissue using ab125208 1 μg/ml.
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Immunocytochemistry - Anti-COL4A2 antibody (ab125208)Immunocytochemistry analysis of A431 cells labeling COL4A2 with ab125208 at 2 µg/mL. Cells incubated with enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with ab125208 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution (green) and incubated for 30 minutes at 37°C. Nuclei visualised using DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse lung tissue using ab125208 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human mammary cancer tissue using ab125208 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat Kidney tissue using ab125208 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat Kidney tissue using ab125208 1 μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125208 has not yet been referenced specifically in any publications.