Anti-CERT antibody (ab72536)
Key features and details
- Rabbit polyclonal to CERT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CERT antibody
See all CERT primary antibodies -
Description
Rabbit polyclonal to CERT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human CERT aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005704.1
Database link: Q9Y5P4-1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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IP |
Use at 1-4 µg/mg of lysate.
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Notes |
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WB
1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
Target
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Function
May mediate the intracellular trafficking of ceramide in a non-vesicular manner. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 PH domain.
Contains 1 START domain. -
Domain
The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB. -
Post-translational
modificationsPhosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. -
Cellular localization
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 286797 Cow
- Entrez Gene: 10087 Human
- Entrez Gene: 100173502 Orangutan
- Entrez Gene: 432007 Xenopus laevis
- Entrez Gene: 550627 Zebrafish
- Entrez Gene: 796455 Zebrafish
- Omim: 604677 Human
- SwissProt: Q9GKI7 Cow
see all -
Alternative names
- Alpha 3 type IV collagen binding protein antibody
- C43BP_HUMAN antibody
- Ceramide transfer protein antibody
see all
Images
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All lanes : Anti-CERT antibody (ab72536) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 100 kDa, 28 kDa, 60 kDa, 70 kDa (possible isoform). We are unsure as to the identity of these extra bands. -
Detection of Human CERT by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab72536 has been referenced in 12 publications.
- Shimasaki K et al. Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum. Int J Mol Sci 23:N/A (2022). PubMed: 35409383
- Goto A et al. Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide. iScience 25:104624 (2022). PubMed: 35800758
- Goto A et al. Involvement of a Cluster of Basic Amino Acids in Phosphorylation-Dependent Functional Repression of the Ceramide Transport Protein CERT. Int J Mol Sci 23:N/A (2022). PubMed: 35955719
- Benedyk TH et al. Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT. J Biol Chem 298:102589 (2022). PubMed: 36243114
- Benedyk TH et al. pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17:e1009824 (2021). PubMed: 34398933