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Rabbit polyclonal to COL4A3BP - N-terminal
HumanPredicted to work with:
Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
Synthetic peptide, corresponding to a region within N terminal amino acids 1-45 of Human COL4A3BP ((Uniprot ID:Q9Y5P4).
- A431, HeLa, HepG2 and H1299 whole cell lysates; methanol-fixed A431 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 68 kDa.
||1/100 - 1/1000.
May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
Contains 1 PH domain.
Contains 1 START domain.
The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB.
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
Information by UniProt
- Alpha 3 type IV collagen binding protein antibody
- C43BP_HUMAN antibody
- Ceramide transfer protein antibody
Western blot - Anti-COL4A3BP antibody - N-terminal (ab151285)
Anti-COL4A3BP antibody - N-terminal (ab151285) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-COL4A3BP antibody - N-terminal (ab151285)
Immunofluorescent analysis of methanol-fixed A431 cells labeling SA1 with ab151285 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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