Key features and details
- Rabbit polyclonal to COL6A1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-COL6A1 antibody
DescriptionRabbit polyclonal to COL6A1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 552-826 of Human COL6A1 (UniProt ID: P12109).
- Mouse brain tissue lysate; Human lung fibroblast tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 109 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCollagen VI acts as a cell-binding protein.
Involvement in diseaseDefects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
Sequence similaritiesBelongs to the type VI collagen family.
Contains 3 VWFA domains.
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
All lanes : Anti-COL6A1 antibody (ab151422) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : COL6A1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 109 kDa
Anti-COL6A1 antibody (ab151422) at 1/500 dilution + mouse brain tissue lysate at 50 µg
Predicted band size: 109 kDa
5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human lung fibroblast tissue labeling COL6A1 with ab151422 at 1/250 dilution.
ab151422 has been referenced in 2 publications.
- Owusu-Ansah KG et al. COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer. Int J Oncol 55:391-404 (2019). PubMed: 31268154
- Du Y et al. Integration of transcriptomic and proteomic data identifies biological functions in cell populations from human infant lung. Am J Physiol Lung Cell Mol Physiol 317:L347-L360 (2019). PubMed: 31268347