Overview

  • Product name

    Anti-COL6A1 antibody
  • Description

    Rabbit polyclonal to COL6A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 552-826 of Human COL6A1 (UniProt ID: P12109).

  • Positive control

    • Mouse brain tissue lysate; Human lung fibroblast tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 109 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Collagen VI acts as a cell-binding protein.
  • Involvement in disease

    Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • Sequence similarities

    Belongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • Collagen alpha-1(VI) chain antibody
    • Collagen antibody
    • Collagen VI, alpha 1 polypeptide antibody
    • Collagen, intimal short chain collagen antibody
    • Collagen, type VI, alpha 1 antibody
    • OPLL antibody
    • OTTHUMP00000115501 antibody
    see all

Images

  • All lanes : Anti-COL6A1 antibody (ab151422) at 1/500 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : COL6A1 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 109 kDa

  • Anti-COL6A1 antibody (ab151422) at 1/500 dilution + mouse brain tissue lysate at 50 µg

    Predicted band size: 109 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human lung fibroblast tissue labeling COL6A1 with ab151422 at 1/250 dilution.

References

ab151422 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151422.
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