• Product name

  • Description

    Rabbit polyclonal to COL8A1
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human COL8A1 aa 600-700 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab117400)

  • Positive control

    • This antibody gave a positive signal in the following Human tissue lysates: Heart; Kidney; Spleen; Liver; Lung.
  • General notes

     This product was previously labelled as Collagen VIII alpha 1




Our Abpromise guarantee covers the use of ab100988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 73 kDa).


  • Function

    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
    Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
  • Tissue specificity

    Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
  • Sequence similarities

    Contains 1 C1q domain.
  • Post-translational

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C3orf7 antibody
    • CO8A1_HUMAN antibody
    • COL8A1 antibody
    • Collagen alpha 1(VIII) chain [Precursor] antibody
    • Endothelial collagen antibody
    • MGC9568 antibody
    • Vastatin antibody
    see all


  • All lanes : Anti-COL8A1 antibody (ab100988) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 73 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes

    The band observed at 62 kDa could potentially be a cleaved form of COL8A1 due to the presence of a 27 amino acid signal peptide. The expression profile observed is consistent with what has been described in the literature (PMID:8921010, PMID:8781485, PMID:10428768, PubMed: 19109829).


This product has been referenced in:

  • Gil-Cayuela C  et al. New Altered Non-Fibrillar Collagens in Human Dilated Cardiomyopathy: Role in the Remodeling Process. PLoS One 11:e0168130 (2016). WB ; Human . Read more (PubMed: 27936202) »
See 1 Publication for this product

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