Anti-COL8A1 antibody (ab58776)

Reviews (1)Q&A (3)References (5)

Key features and details

  • Rabbit polyclonal to COL8A1
  • Suitable for: ELISA, WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-COL8A1 antibody
    See all COL8A1 primary antibodies

  • Description

    Rabbit polyclonal to COL8A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rabbit, Human

  • Immunogen

    Synthetic peptide corresponding to Human COL8A1 (C terminal).
    (Peptide available as ab138129)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab58776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Can be blocked with COL8A1 peptide (ab138129).
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Can be blocked with COL8A1 peptide (ab138129).

Target

  • Function

    Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
    Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.

  • Tissue specificity

    Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.

  • Sequence similarities

    Contains 1 C1q domain.

  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C3orf7 antibody
    • CO8A1_HUMAN antibody
    • COL8A1 antibody
    • Collagen alpha 1(VIII) chain [Precursor] antibody
    • Endothelial collagen antibody
    • MGC9568 antibody
    • Vastatin antibody
    see all

References (5)

Publishing research using ab58776? Please let us know so that we can cite the reference in this datasheet.

ab58776 has been referenced in 5 publications.

  • Guo W  et al. Screening and bioinformatical analysis of differentially expressed genes in nasopharyngeal carcinoma. J Cancer 12:1867-1883 (2021). PubMed: 33753985
  • Ma ZH  et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . PubMed: 23170115
  • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . PubMed: 21372207
  • Todorovic V  et al. Detection of differentially expressed basal cell proteins by mass spectrometry. Mol Cell Proteomics 9:351-61 (2010). WB ; Mouse . PubMed: 19955077
  • Zhao Y  et al. siRNA-targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D-limonence treatment in hepatocarcinoma cells. IUBMB Life 61:74-9 (2009). WB ; Mouse . PubMed: 19109829

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-COL8A1 antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231, MCF-7)
Gel Running Conditions
Reduced Denaturing (10-20% tris-glycine)
Loading amount
35 µg
Specification
MDA-MB-231, MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Mar 04 2019

Question

Thank you for getting back to me. If you could let me know if there are any changes to the status of the peptide that would be great.

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Answer

Yes, I will let you know.

Thank you so much for your patience and understanding!

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Question

peptide available for sale?

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Answer

Thank you for contacting us.

I have good news: the peptide is available for sale, but is currently backordered for 3 weeks.
Once I have more information about the catalog number and datasheet I will let you know - hopefully by the end of the week.

Thank you for your patience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir or Madame, I am working with your col8a1 antibody (ab58776). Is this antibody tested for crossreaction with the alpha 2 chain of collagen VIII, as I have the feeling that some of my results in Western blotting could rather be the alpha 2 than the alpha 1 chain of collagen VIII. Thank you in advance for your answer. Yours sincerely

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Answer

Thank you for your inquiry.  A cross-reaction of this antibody with the collagen alpha2 (VIII) chain was not specifically tested. But I can confirm that a cross-reaction is very unlikely because the immunogen sequence only shares 66% homology with alpha2. We expect a possible cross-reaction from 85% homology and more. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.  

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