Anti-COL8A1 antibody (ab58776)

Reviews (1)Q&A (3)References (4)

Overview

  • Product name

    Anti-COL8A1 antibody
    See all COL8A1 primary antibodies

  • Description

    Rabbit polyclonal to COL8A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rabbit, Human

  • Immunogen

    Synthetic peptide corresponding to Human COL8A1 (C terminal).
    (Peptide available as ab138129)

  • General notes

     This product was previously labelled as Collagen VIII alpha 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab58776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
      Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.

    • Tissue specificity

      Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.

    • Sequence similarities

      Contains 1 C1q domain.

    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C3orf7 antibody
      • CO8A1_HUMAN antibody
      • COL8A1 antibody
      • Collagen alpha 1(VIII) chain [Precursor] antibody
      • Endothelial collagen antibody
      • MGC9568 antibody
      • Vastatin antibody
      see all

    References

    This product has been referenced in:

    • Ma ZH  et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . Read more (PubMed: 23170115) »
    • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . Read more (PubMed: 21372207) »
    See all 4 Publications for this product

    Publishing research using ab58776? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-COL8A1 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB-231, MCF-7)
    Gel Running Conditions
    Reduced Denaturing (10-20% tris-glycine)
    Loading amount
    35 µg
    Specification
    MDA-MB-231, MCF-7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2019

    Question

    Thank you for getting back to me. If you could let me know if there are any changes to the status of the peptide that would be great.

    Read More
    Answer

    Yes, I will let you know.

    Thank you so much for your patience and understanding!

    Read More

    Question

    peptide available for sale?

    Read More
    Answer

    Thank you for contacting us.

    I have good news: the peptide is available for sale, but is currently backordered for 3 weeks.
    Once I have more information about the catalog number and datasheet I will let you know - hopefully by the end of the week.

    Thank you for your patience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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    Question

    Dear Sir or Madame, I am working with your col8a1 antibody (ab58776). Is this antibody tested for crossreaction with the alpha 2 chain of collagen VIII, as I have the feeling that some of my results in Western blotting could rather be the alpha 2 than the alpha 1 chain of collagen VIII. Thank you in advance for your answer. Yours sincerely

    Read More
    Answer

    Thank you for your inquiry.  A cross-reaction of this antibody with the collagen alpha2 (VIII) chain was not specifically tested. But I can confirm that a cross-reaction is very unlikely because the immunogen sequence only shares 66% homology with alpha2. We expect a possible cross-reaction from 85% homology and more. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.  

    Read More

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