Anti-COL8A1 antibody (ab58776)
Key features and details
- Rabbit polyclonal to COL8A1
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-COL8A1 antibody
See all COL8A1 primary antibodies -
Description
Rabbit polyclonal to COL8A1 -
Host species
Rabbit -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rabbit, Human -
Immunogen
Synthetic peptide corresponding to Human COL8A1 (C terminal).
(Peptide available asab138129) -
General notes
This product was previously labelled as Collagen VIII alpha 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
WB: Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis. -
Tissue specificity
Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 1295 Human
- Entrez Gene: 12837 Mouse
- Entrez Gene: 100009383 Rabbit
- Omim: 120251 Human
- SwissProt: P27658 Human
- SwissProt: Q00780 Mouse
- SwissProt: P14282 Rabbit
- Unigene: 654548 Human
see all -
Alternative names
- C3orf7 antibody
- CO8A1_HUMAN antibody
- COL8A1 antibody
see all
Datasheets and documents
References (4)
ab58776 has been referenced in 4 publications.
- Ma ZH et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . PubMed: 23170115
- Loeffler I et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . PubMed: 21372207
- Todorovic V et al. Detection of differentially expressed basal cell proteins by mass spectrometry. Mol Cell Proteomics 9:351-61 (2010). WB ; Mouse . PubMed: 19955077
- Zhao Y et al. siRNA-targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D-limonence treatment in hepatocarcinoma cells. IUBMB Life 61:74-9 (2009). WB ; Mouse . PubMed: 19109829