For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    col8a1-antibody-ab58776.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
Share by email

Anti-COL8A1 antibody (ab58776)

  • Datasheet
Reviews (1)Q&A (3)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit polyclonal to COL8A1
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-Collagen VI antibody [EPR17072] (ab182744)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-Collagen VII antibody (ab223639)

View more associated products

Overview

  • Product name

    Anti-COL8A1 antibody
    See all COL8A1 primary antibodies
  • Description

    Rabbit polyclonal to COL8A1
  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human COL8A1 (C terminal).
    (Peptide available as ab138129)

  • General notes

     This product was previously labelled as Collagen VIII alpha 1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Extracellular matrix

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • COL8A1 peptide (ab138129)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
      Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
    • Tissue specificity

      Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
    • Sequence similarities

      Contains 1 C1q domain.
    • Post-translational
      modifications

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Target information above from: UniProt accession P27658 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1295 Human
      • Entrez Gene: 12837 Mouse
      • Entrez Gene: 100009383 Rabbit
      • Omim: 120251 Human
      • SwissProt: P27658 Human
      • SwissProt: Q00780 Mouse
      • SwissProt: P14282 Rabbit
      • Unigene: 654548 Human
      • Unigene: 130388 Mouse
      see all
    • Alternative names

      • C3orf7 antibody
      • CO8A1_HUMAN antibody
      • COL8A1 antibody
      • Collagen alpha 1(VIII) chain [Precursor] antibody
      • Endothelial collagen antibody
      • MGC9568 antibody
      • Vastatin antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab58776? Please let us know so that we can cite the reference in this datasheet.

    ab58776 has been referenced in 4 publications.

    • Ma ZH  et al. Effect of enhanced expression of COL8A1 on lymphatic metastasis of hepatocellular carcinoma in mice. Exp Ther Med 4:621-626 (2012). WB . PubMed: 23170115
    • Loeffler I  et al. Type VIII Collagen Modulates TGF-{beta}1-induced Proliferation of Mesangial Cells. J Am Soc Nephrol 22:649-63 (2011). WB ; Mouse . PubMed: 21372207
    • Todorovic V  et al. Detection of differentially expressed basal cell proteins by mass spectrometry. Mol Cell Proteomics 9:351-61 (2010). WB ; Mouse . PubMed: 19955077
    • Zhao Y  et al. siRNA-targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D-limonence treatment in hepatocarcinoma cells. IUBMB Life 61:74-9 (2009). WB ; Mouse . PubMed: 19109829

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-COL8A1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB-231, MCF-7)
    Gel Running Conditions
    Reduced Denaturing (10-20% tris-glycine)
    Loading amount
    35 µg
    Specification
    MDA-MB-231, MCF-7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2019

    Question

    Thank you for getting back to me. If you could let me know if there are any changes to the status of the peptide that would be great.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 30 2012

    Answer

    Yes, I will let you know.

    Thank you so much for your patience and understanding!

    Read More

    Abcam Scientific Support

    Answered on Jul 30 2012

    Question

    peptide available for sale?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2012

    Answer

    Thank you for contacting us.

    I have good news: the peptide is available for sale, but is currently backordered for 3 weeks.
    Once I have more information about the catalog number and datasheet I will let you know - hopefully by the end of the week.

    Thank you for your patience.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 06 2012

    Question

    Dear Sir or Madame, I am working with your col8a1 antibody (ab58776). Is this antibody tested for crossreaction with the alpha 2 chain of collagen VIII, as I have the feeling that some of my results in Western blotting could rather be the alpha 2 than the alpha 1 chain of collagen VIII. Thank you in advance for your answer. Yours sincerely

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2011

    Answer

    Thank you for your inquiry.  A cross-reaction of this antibody with the collagen alpha2 (VIII) chain was not specifically tested. But I can confirm that a cross-reaction is very unlikely because the immunogen sequence only shares 66% homology with alpha2. We expect a possible cross-reaction from 85% homology and more. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.  

    Read More

    Abcam Scientific Support

    Answered on Oct 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.