Anti-Collagen alpha-4(IV) chain antibody (ab193951)
Key features and details
- Goat polyclonal to Collagen alpha-4(IV) chain
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-Collagen alpha-4(IV) chain antibody -
Description
Goat polyclonal to Collagen alpha-4(IV) chainThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Goat -
Species reactivity
Predicted to work with: Human -
Immunogen
Synthetic peptide corresponding to Human Collagen alpha-4(IV) chain aa 1550-1650 (internal sequence) (Cysteine residue). NP_000083.3.
Database link: P53420 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab193951 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 164 kDa.
A 1 hour primary incubation is recommended for this product. Preliminary experiments in lysates of several human tissues and cell lines gave no specific signal but low background (at antibody concentration up to 1µg/ml), we would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 164 kDa. A 1 hour primary incubation is recommended for this product. Preliminary experiments in lysates of several human tissues and cell lines gave no specific signal but low background (at antibody concentration up to 1µg/ml), we would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Target
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Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. -
Tissue specificity
Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. Highest levels of expression of alpha 4 type IV collagen are detected in kidney, calvaria, neuroretina and cardiac muscle. Lower levels of expression are observed in brain, lung and thymus, and no expression is detected in choroid plexus, liver, adrenal, pancreas, ileum or skin. -
Involvement in disease
Alport syndrome, autosomal recessive
Hematuria, benign familial -
Sequence similarities
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
Domain
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL). - Information by UniProt
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Database links
- Entrez Gene: 1286 Human
- SwissProt: P53420 Human
- Unigene: 591645 Human
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Alternative names
- CO4A4_HUMAN antibody
- COL4A4 antibody
- Collagen alpha-4(IV) chain antibody
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab193951 has not yet been referenced specifically in any publications.