For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I am looking for an antibody that will bind to porcine collagen and NOT rat collagen for use in an immunohistochemistry/ immunofluorescense experiment. Would this antibody be appropriate? If not, do you have any recommendations?
Asked on Aug 08 2012
ab24135 is unlikely to be a good choice for this purpose, though it is raised against pig collagen. It will be difficult to find anti-collagen antibodies that are reactive with one species and not another, given that collagens are well-conserved between rat and pig. Rat reactivity is not listed for ab24135 because it has not been tested against rat samples, but in general, polyclonal anti-collagen antibodies are reactive with a broad range of species.
If your collagen is expected to contain the procollagen region (intracellular, not secreted), then you should consider ab64409. Although reactivity with rat collagen has not been assessed, there is less conservation in this region. However, ab64409 is raised in rat, so a secondary antibody against rat IgG may react with endogenous rat IgG, if your samples are rat tissue.
Answered on Aug 08 2012