• Product name

    Anti-Collagen I + Collagen III antibody
    See all Collagen I + Collagen III primary antibodies
  • Description

    Rabbit polyclonal to Collagen I + Collagen III
  • Host species

  • Specificity

    Specificity was ascertained by direct ELISA using pure dog collagen types I and III for coating microplate wells. No binding to canine serum proteins is revealed at similar dilutions of the antibody.
  • Tested applications

    Suitable for: ELISA, WB, ICC, IHC-Frmore details
  • Species reactivity

    Reacts with: Dog
  • Immunogen

    Full length native protein (purified) (Dog). Extensively purified native collagen type I and III extracted from dog tail tendon.

  • Positive control

    • Native dog collagen type I and III for dot blotting and ELISA.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: The antibody was extracted into dilute acidic buffer after mild pepsin digestion.
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Pooled antisera are passed over DEAE cellulose to produce IgG enriched fraction. The affinity purified antibody is obtained by binding to immobilized native dog collagen, mixture of type I and III (the antigens used for immunization), followed by elution with acidic buffer, neutralisation, dialysis, dispensing and lyophilization.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab24137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution. The antibody can be diluted at least 1/20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC labeled secondary antibody is used the antibody can be diluted 1/10.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
    • Cellular localization

    • Alternative names

      • Alpha 1 type I collagen antibody
      • Alpha 2 type I collagen antibody
      • COL1A1 antibody
      • COL1A2 antibody
      • COL3A1 antibody
      • Collagen fetal antibody
      • Collagen type I alpha 1 antibody
      • Collagen type I alpha 2 antibody
      • Collagen type III alpha 1 antibody
      • OI4 antibody
      • Type I collagen alpha 1 chain antibody
      • Type I procollagen antibody
      see all


    This product has been referenced in:

    • Liu MM  et al. Development and Evaluation of a Tissue-Engineered Fibrin-based Canine Mitral Valve Three-dimensional Cell Culture System. J Comp Pathol 160:23-33 (2018). WB, IHC-P ; Dog . Read more (PubMed: 29729718) »
    • Sivasubramanian S  et al. Phenotypic Screening Identifies Synergistically Acting Natural Product Enhancing the Performance of Biomaterial Based Wound Healing. Front Pharmacol 8:433 (2017). WB . Read more (PubMed: 28769790) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Western blot
    Mouse Tissue lysate - whole (Hippocampus)
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Aug 16 2010

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