Overview

  • Product name
    Anti-Collagen I + II + III + IV + V antibody
    See all Collagen I + II + III + IV + V primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + II + III + IV + V
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Constituents: 0.1% Dextran, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.1% Mannitol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/40.
ELISA 1/2000 - 1/8000.
IHC-Fr 1/10 - 1/40.

Target

  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Caffey disease
    Ehlers-Danlos syndrome, classic type
    Ehlers-Danlos syndrome 7A
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    Osteoporosis
    A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Domain
    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • EDS4A antibody
    see all

References

This product has been referenced in:
  • Schwenk R  et al. The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells. Oncotarget 8:77622-77633 (2017). Read more (PubMed: 29100413) »
  • Sur S  et al. Synergistic regulation of cerebellar Purkinje neuron development by laminin epitopes and collagen on an artificial hybrid matrix construct. Biomater Sci 2:903-914 (2014). Read more (PubMed: 25530849) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
300 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12% precast)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 15 2012

Answer

Thank you for your reply. Below is the discount for testing ab36064.

Please let me know if there is anything else I can help you with.

DISCOUNT CODE: *********
Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab36064 in western blot. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forwestern blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting Abcam.

The testing discount codes are normally issued before you purchase the antibody, this is so we can track it in our system. However in this case I will make an exception and issue the code post purchase. To do this, could you please provide me with the Abcam order number for ab36064, so that I can generate a code.

I look forward to your reply and issuing the code.

Read More

Answer

Thank you for contacting Abcam.
I have spoken to the lab about the epitopes that are recognized by ab36064 and the epitope has not been mapped for this antibody and because it is a polyclonal antibody, it will recognize multiple epitopes.
I believe it would be suitable for your needs since it does recognize multiply collagen family members. As to whether using this antibody under reducing conditions would affect it, I cannot say for certain, but I do not think it will. Although since we have not tested this antibody in western blot, we cannot guarantee that it will work, therefore you could take advantage of our testing discount program:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Please let me know if there is anything else I can help you with.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up