Anti-Collagen I + II + III + IV + V antibody (ab36064)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Collagen I + II + III + IV + V antibody
See all Collagen I + II + III + IV + V primary antibodies -
Description
Rabbit polyclonal to Collagen I + II + III + IV + V -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.1% Dextran, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.1% Mannitol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab36064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/10 - 1/40. | |
ELISA | 1/2000 - 1/8000. | |
IHC-Fr | 1/10 - 1/40. |
Target
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Caffey disease
Ehlers-Danlos syndrome, classic type
Ehlers-Danlos syndrome 7A
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
Osteoporosis
A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1277 Human
- Entrez Gene: 1280 Human
- Entrez Gene: 1281 Human
- Entrez Gene: 1282 Human
- Entrez Gene: 1289 Human
- Omim: 120130 Human
- Omim: 120140 Human
- Omim: 120150 Human
see all -
Alternative names
- Alpha-1 type I collagen antibody
- CO1A1_HUMAN antibody
- COL1A1 antibody
see all
References
This product has been referenced in:
- Luong B et al. The vacuolar-type ATPase inhibitor archazolid increases tumor cell adhesion to endothelial cells by accumulating extracellular collagen. PLoS One 13:e0203053 (2018). Read more (PubMed: 30204757) »
- Schwenk R et al. The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells. Oncotarget 8:77622-77633 (2017). Read more (PubMed: 29100413) »