Overview

  • Product name

    Anti-Collagen I + II + III + IV + V antibody
    See all Collagen I + II + III + IV + V primary antibodies
  • Description

    Rabbit polyclonal to Collagen I + II + III + IV + V
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.50
    Constituents: 0.1% Dextran, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.1% Mannitol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/40.
ELISA 1/2000 - 1/8000.
IHC-Fr 1/10 - 1/40.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease

    Caffey disease
    Ehlers-Danlos syndrome, classic type
    Ehlers-Danlos syndrome 7A
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    Osteoporosis
    A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Domain

    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • EDS4A antibody
    see all

References

This product has been referenced in:

  • Luong B  et al. The vacuolar-type ATPase inhibitor archazolid increases tumor cell adhesion to endothelial cells by accumulating extracellular collagen. PLoS One 13:e0203053 (2018). Read more (PubMed: 30204757) »
  • Schwenk R  et al. The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells. Oncotarget 8:77622-77633 (2017). Read more (PubMed: 29100413) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
300 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12% precast)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 15 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up