Question (46922) | Anti-Collagen I + II + III + IV + V antibody (ab36064)

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Question

I am interested in a non-specific antibody for immunoprecipitating collagen peptides from human serum. Can you give me your impressions on whether this antibody is suitable? Do you know what the epitope(s) are, and whether the antibody binding depends on the collagen having an intact triple helix structure? Along those lines, on a western blot, would reducing vs. non-reducing conditions affect the detection?

Answer

Thank you for contacting Abcam.
I have spoken to the lab about the epitopes that are recognized by ab36064 and the epitope has not been mapped for this antibody and because it is a polyclonal antibody, it will recognize multiple epitopes.
I believe it would be suitable for your needs since it does recognize multiply collagen family members. As to whether using this antibody under reducing conditions would affect it, I cannot say for certain, but I do not think it will. Although since we have not tested this antibody in western blot, we cannot guarantee that it will work, therefore you could take advantage of our testing discount program:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Please let me know if there is anything else I can help you with.

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