Anti-Collagen I antibody [3G3] (ab88147)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-218359-anti-collagen-i-antibody-3g3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)")
Key features and details
- Mouse monoclonal [3G3] to Collagen I
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG3
Overview
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Product name
Anti-Collagen I antibody [3G3]
See all Collagen I primary antibodies -
Description
Mouse monoclonal [3G3] to Collagen I -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB MouseRecombinant fragment
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Immunogen
Recombinant fragment (GST-tag) corresponding to Human Collagen I aa 1021-1109.
Sequence:EGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETG PAGPAGPVGPVGARGPAGPQGPRGDKGETGEQGDRGIK
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Positive control
- NIH 3T3 cell lysate IHC-P: FFPE human normal skin, human normal placenta, mouse normal placenta.
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General notes
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
3G3 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
Human
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| WB |
Mouse
Recombinant fragment
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.
Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (6) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa. Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1277 Human
- Entrez Gene: 1278 Human
- Entrez Gene: 12842 Mouse
- Entrez Gene: 12843 Mouse
- Omim: 120150 Human
- Omim: 120160 Human
- SwissProt: P02452 Human
- SwissProt: P08123 Human
see all -
Alternative names
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- alpha 2 type I procollagen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
IHC-P of ab88147 on human pancreas. antibody concentration 3 ug/ml.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-259004-anti-collagen-i-antibody-3g3-western-blot.jpg)
Western blot - Anti-Collagen I antibody [3G3] (ab88147)All lanes : Anti-Collagen I antibody [3G3] (ab88147) at 1/1000 dilution
Lane 1 : Mouse skin
Lane 2 : Mouse placenta
Lane 3 : Mouse kidney
Lane 4 : Mouse liver
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR- goat anti-mouse (green) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 139 kDaThis blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with mouse anti-Collagen I antibody (88147; 1:1000) and the loading control rabbit anti-GAPDH antibody (ab9485; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-mouse (green; 1:10000) and IR-goat anti-rabbit (red; 1:10000) for 1 hour at room temperature before imaging.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-258893-anti-collagen-i-antibody-3g3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)IHC image of collagen I staining in a section of normal mouse placenta formalin-fixed paraffin-embedded. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab88147 at 1/200 dilution. An HRP-conjugated secondary (Ab97040, 1/500 dilution) was used for 1hr at room temperature. DAB was used as the chromogen (10min at room temperature). The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-103142-anti-collagen-i-antibody-3g3-western-blot.jpg)
Western blot - Anti-Collagen I antibody [3G3] (ab88147)Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + immunogen (88 aa recombinant fragment with a proprietary tag of 26kDa) at 0.2 µg
Secondary
Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 139 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-258844-anti-collagen-i-antibody-3g3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)IHC image of Collagen I staining in normal human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This image was generated using the ascites version of the product.
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![Western blot - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-103141-anti-collagen-i-antibody-3g3-western-blot.jpg)
Western blot - Anti-Collagen I antibody [3G3] (ab88147)Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + NIH 3T3 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 139 kDaThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-103140-anti-collagen-i-antibody-3g3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)IHC image of Collagen I staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This image was generated using the ascites version of the product.
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![ELISA - Anti-Collagen I antibody [3G3] (ab88147)](https://www.abcam.com/ps/products/88/ab88147/Images/ab88147-218357-anti-collagen-i-antibody-3g3-elisa.jpg)
ELISA - Anti-Collagen I antibody [3G3] (ab88147)Detection limit for ab88147 is 0.03 ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab88147 has been referenced in 24 publications.
- Lei X et al. The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies. Cell Adh Migr 14:12-23 (2020). PubMed: 31942821
- Huang L et al. Enriched LPS Staining within the Germinal Center of a Lymph Node from an HIV-Infected Long-Term Nonprogressor but Not from Progressors. J Immunol Res 2020:7471380 (2020). PubMed: 32455142
- Cavalcante MB et al. Dasatinib plus quercetin prevents uterine age-related dysfunction and fibrosis in mice. Aging (Albany NY) 12:2711-2722 (2020). PubMed: 31955151
- Vieira JS et al. Alendronate induces postnatal maxillary bone growth by stimulating intramembranous ossification and preventing premature cartilage mineralization in the midpalatal suture of newborn rats. Int J Oral Maxillofac Surg N/A:N/A (2019). PubMed: 31054875
- Pan J et al. Pterostilbene, a bioactive component of blueberries, alleviates renal fibrosis in a severe mouse model of hyperuricemic nephropathy. Biomed Pharmacother 109:1802-1808 (2019). PubMed: 30551434