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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Anti-Collagen I antibody [3G3] (ab88147)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (24)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
  • ELISA - Anti-Collagen I antibody [3G3] (ab88147)

Key features and details

  • Mouse monoclonal [3G3] to Collagen I
  • Suitable for: WB, ELISA, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG3

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Overview

  • Product name

    Anti-Collagen I antibody [3G3]
    See all Collagen I primary antibodies
  • Description

    Mouse monoclonal [3G3] to Collagen I
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human Collagen I aa 1021-1109.
    Sequence:

    EGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETG PAGPAGPVGPVGARGPAGPQGPRGDKGETGEQGDRGIK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • NIH 3T3 cell lysate IHC-P: FFPE human normal skin, human normal placenta, mouse normal placenta.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    3G3
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Collagen I protein (ab158152)
  • Related Products

    • Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
    • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
    • Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216791)
    • Native Cow Collagen I protein (ab7526)
    • Native Human Collagen I protein (ab7533)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
WB
Mouse
Recombinant fragment
Application Abreviews Notes
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

ELISA
Use at an assay dependent concentration.
IHC-P (6)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease

    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1277 Human
    • Entrez Gene: 1278 Human
    • Entrez Gene: 12842 Mouse
    • Entrez Gene: 12843 Mouse
    • Omim: 120150 Human
    • Omim: 120160 Human
    • SwissProt: P02452 Human
    • SwissProt: P08123 Human
    • SwissProt: P11087 Mouse
    • SwissProt: Q01149 Mouse
    • Unigene: 172928 Human
    • Unigene: 489142 Human
    • Unigene: 681002 Human
    • Unigene: 277735 Mouse
    • Unigene: 277792 Mouse
    • Unigene: 458212 Mouse
    see all
  • Alternative names

    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)

    IHC-P of ab88147 on human pancreas. antibody concentration 3 ug/ml.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    All lanes : Anti-Collagen I antibody [3G3] (ab88147) at 1/1000 dilution

    Lane 1 : Mouse skin
    Lane 2 : Mouse placenta
    Lane 3 : Mouse kidney
    Lane 4 : Mouse liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IR- goat anti-mouse (green) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 139 kDa



    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with mouse anti-Collagen I antibody (88147; 1:1000) and the loading control rabbit anti-GAPDH antibody (ab9485; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-mouse (green; 1:10000) and IR-goat anti-rabbit (red; 1:10000) for 1 hour at room temperature before imaging.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)

    IHC image of collagen I staining in a section of normal mouse placenta formalin-fixed paraffin-embedded. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab88147 at 1/200 dilution. An HRP-conjugated secondary (Ab97040, 1/500 dilution) was used for 1hr at room temperature. DAB was used as the chromogen (10min at room temperature). The section was counterstained with haematoxylin and mounted with DPX. 

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + immunogen (88 aa recombinant fragment with a proprietary tag of 26kDa) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 139 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)

    IHC image of Collagen I staining in normal human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    Western blot - Anti-Collagen I antibody [3G3] (ab88147)
    Anti-Collagen I antibody [3G3] (ab88147) at 1 µg/ml + NIH 3T3 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 139 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [3G3] (ab88147)

    IHC image of Collagen I staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88147, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

  • ELISA - Anti-Collagen I antibody [3G3] (ab88147)
    ELISA - Anti-Collagen I antibody [3G3] (ab88147)

    Detection limit for ab88147 is 0.03 ng/ml as a capture antibody.

    This image was generated using the ascites version of the product.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (24)

Publishing research using ab88147? Please let us know so that we can cite the reference in this datasheet.

ab88147 has been referenced in 24 publications.

  • Lei X  et al. The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies. Cell Adh Migr 14:12-23 (2020). PubMed: 31942821
  • Huang L  et al. Enriched LPS Staining within the Germinal Center of a Lymph Node from an HIV-Infected Long-Term Nonprogressor but Not from Progressors. J Immunol Res 2020:7471380 (2020). PubMed: 32455142
  • Cavalcante MB  et al. Dasatinib plus quercetin prevents uterine age-related dysfunction and fibrosis in mice. Aging (Albany NY) 12:2711-2722 (2020). PubMed: 31955151
  • Vieira JS  et al. Alendronate induces postnatal maxillary bone growth by stimulating intramembranous ossification and preventing premature cartilage mineralization in the midpalatal suture of newborn rats. Int J Oral Maxillofac Surg N/A:N/A (2019). PubMed: 31054875
  • Pan J  et al. Pterostilbene, a bioactive component of blueberries, alleviates renal fibrosis in a severe mouse model of hyperuricemic nephropathy. Biomed Pharmacother 109:1802-1808 (2019). PubMed: 30551434
View all Publications for this product

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen I antibody [3G3]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3 cells)
Permeabilization
No
Specification
NIH 3T3 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Methanol
Read More

Dr. Blanca Arnaiz

Verified customer

Submitted Dec 07 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mesenchymal tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Mesenchymal tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

Submitted Mar 13 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

Submitted Mar 13 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 13 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (condyl)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
condyl
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2400µg/mL · Temperature: 24°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 18 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (condyl)
Antigen retrieval step
Other
Permeabilization
No
Specification
condyl
Blocking step
goat serum as blocking agent for 30 minute(s) · Concentration: 2400µg/mL · Temperature: 24°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 11 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen I antibody [3G3]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Skin
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 15 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Collagen I antibody [3G3]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Guinea pig Tissue sections (Skin, Epidermis)
Permeabilization
No
Specification
Skin, Epidermis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Karnovskys
Read More

Prof. Kent Bachus

Verified customer

Submitted Nov 21 2015

Immunoprecipitation abreview for Anti-Collagen I antibody [3G3]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - other (Serum)
Specification
Serum
Total protein in input
5 µg
Read More

Abcam user community

Verified customer

Submitted Jul 23 2014

Western blot abreview for Anti-Collagen I antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Tissue lysate - whole (ear section)
Specification
ear section
Blocking step
IRDye blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 06 2013

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